Role of P38 mitogen-activated protein kinases signaling pathway in the differentiation of bone morphogenetic protein 9 induced C3H10T1/2 stem cell into myocardiocytes

ZHANG Fen; CHEN Yuan; SUN Wenjing; CHEN Lu; CHEN Miaoyue; ZHU Gaohui

您当前的位置：

首页 >

文章列表页 >

Role of P38 mitogen-activated protein kinases signaling pathway in the differentiation of bone morphogenetic protein 9 induced C3H10T1/2 stem cell into myocardiocytes

更新时间：2023-12-18

- Role of P38 mitogen-activated protein kinases signaling pathway in the differentiation of bone morphogenetic protein 9 induced C3H10T1/2 stem cell into myocardiocytes
- Journal of Chongqing Medical University Vol. 37, Issue 8, Pages: 657-660(2012)
- 作者机构：
  
  重庆医科大学附属儿童医院心血管内科,重庆,400014
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2012，
扫描看全文
ZHANG Fen, CHEN Yuan, SUN Wenjing, et al. Role of P38 mitogen-activated protein kinases signaling pathway in the differentiation of bone morphogenetic protein 9 induced C3H10T1/2 stem cell into myocardiocytes. [J]. Journal of Chongqing Medical University 37(8):657-660(2012)
DOI：

ZHANG Fen, CHEN Yuan, SUN Wenjing, et al. Role of P38 mitogen-activated protein kinases signaling pathway in the differentiation of bone morphogenetic protein 9 induced C3H10T1/2 stem cell into myocardiocytes. [J]. Journal of Chongqing Medical University 37(8):657-660(2012) DOI：

摘要

目的：研究P38丝裂原活化蛋白激酶（P38 mitogen-activated protein kinases，P38MAPK）通路在骨形态发生蛋白9（Bone morphogenetic protein 9，BMP9）诱导 C3H10T1/2干细胞向心肌样细胞分化中的作用。方法：免疫印迹检测经pAdEasyBMP9诱导24 h的C3H10T1/2干细胞磷酸化P38MAPK和P38MAPK表达水平，免疫荧光定位磷酸化P38MAPK；SB203580抑制C3H10T1/2干细胞磷酸化P38MAPK活性，pAdEasyBMP9诱导21 d时免疫印迹检测心肌特异性蛋白CX43、cTnT表达，免疫荧光检测心肌特异性蛋白cTnT、α-MHC表达，RT-qPCR检测心肌特异性基因GATA4、MEF2C表达。结果：pAdEasyBMP9促进磷酸化P38MAPK表达增高，却不影响P38MAPK表达水平；磷酸化P38MAPK有表达于胞浆和胞核、仅表达于胞核的不同状态。SB203580可显著性地抑制由pAdEasyBMP9诱导的C3H10T1/2干细胞CX43、cTnT、α-MHC、GATA4、MEF2C表达。结论：pAdEasyBMP9诱导C3H10T1/2干细胞能激活P38MAPK通路，并促进其向心肌样细胞分化。

Abstract

Objective：To investigate the role of P38 mitogen-activated protein kinases（P38MAPK） signal pathway in the differentiation of bone morphogenetic protein 9（BMP9） induced C3H10T1/2 stem cell into myocardiocytes. Methods：The phospho-P38MAPK and P38MAPK were measured by Western blot at 24 h after the C3H10T1/2 stem cell being transfected with pAdEasyBMP9 and the phos－pho-P38MAPK was detected by immunofluorescence at 24 h after the C3H10T1/2 stem cell being transfected with pAdEasyBMP9. The cardiac-specific proteins cTnT and CX43 were measured by Western blot，the cardiac-specific proteins cTnT and α-MHC were measured by immunofluorescence，the cardiac-specific gene GATA4 and MEF2C expressions were detected by RT-qPCR at 21 d after the C3H10T1/2 stem cell being transfected with pAdEasyBMP9 and treated with SB203580. Results：Transfection with pAdEasyBMP9 produced rapid increase in phospho-P38MAPK and little change in P38MAPK. Phospho-P38MAPK expressed in nucleus and cytoplasm or in different states of nucleus. SB203580 significantly inhibited the pAdEasyBMP9-induced expression of CX43，cTnT，α-MHC，GATA4，MEF2C in C3H10T1/2 stem cell. Conclusions：P38MAPK signal pathway can be activated in C3H10T1/2 stem cell after being transfected with pAdEasyBMP9 and can promote the myocardiocytes differentiation.

关键词

骨形态发生蛋白9C3H10T1/2干细胞心肌样细胞P38丝裂原活化蛋白激酶

Keywords

bone morphogenetic protein 9C3H10T1/2 stem cellcardiomyocytesP38 mitogen-activated protein kinases

references

Views

1059

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Role of FSHβ in BMP9-induced osteogenic differentiation in mouse embryonic fibroblasts

Notch pathway inhibits BMP9-induced osteogenic differentiation of mesenchymal stem cells C3H10T1/2 by down-regulating expression of gene JunB

Notch pathway inhibits BMP9-induced osteogenic differentiation of mesenchymal stem cells C3H10T1/2 by down-regulating

Mechanism of osteogenic differentiation for mesenchymal stem cells induced by BMP9 and Wnt3a in vitro

Effects of Dkk1 on bone morphogenetic protein 9 induced mesenchymal stem cells osteogenic differentiation

Related Author

Liu Hong

Huang Min

Su Xiaoya

Luo Xiaoji

Lin Duanyang

Tan Tao

Wang Nan

LIANG Xi

Related Institution

Department of Reproduction Health and Infertility,The First Affiliated Hospital of Chongqing Medical University

First Medical College of Chongqing Medical University

Department of Orthopedics，The First Affiliated Hospital of Chongqing Medical University

Department of Orthopedics，the First Affiliated Hospital

Key Laboratory of Clinical Laboratory Diagnostics，Chongqing Medical Uninversity

⁰