Objective：To observe the relation between the expression of EGF-R or IGF-1-R and epithelial differentiation in bone marrow mesenchymal stem cells（BMSCs）. Methods：The cultured passage 3（P3） BMSCs were induced or not induced by EGF or IGF-1；The P5-BMSCs were cultured with antibody of EGF-R or IGF-1-R for 4 h，and then induced or not induced by EGF or IGF-1.The expression time of EGF-R，IGF-1-R and CK in these cells was detected with immunohistochemical Sp method. Results：The first expression time of EGF-R，IGF-1-R and CK in un-induced P3-BMSCs was respectively on the 8th～11th d，10th～14th d and 16th～18th d. The expression time of EGF-R or IGF-1-R，which was induced by EGF or IGF-1，was 2 d～4 d earlier than the time in un-in－duced cells. When P5-BMSCs were blocked by antibody of EGF-R or IGF-1-R，the expression time of CK was 2 days later than that of control. Conclusion：The expression of EGF-R and IGF-1-R in BMSCs was closely correlated with their epithelial differentiation in vitro；The passages 4～5 BMSCs were best time for the addition of exogenous EGF、IGF-1 to induce BMSCs differentiation into ep－ithelial cell in vitro.