Objective：To exam the serum anti-survivin antibody levels in post-surgery and pre-surgery samples of patients with laryngeal squamous cell carcinoma（LSCC） and to investigate the potential use of anti-survivin，anti-p53，and anti-c-myc antibodies in the monitoring of LSCC postoperatively. Methods：Enzyme-linked immunosorbent assay（ELISA） was used for the detection of serum anti-survivin，anti-p53，and anti-c-myc antibodies. Results：（1）After radical surgical treatment，the anti-survivin，anti-p53，and anti-c-myc antibodies expression levels were decreased significantly. （2）High levels of anti-survivin antibody were detected in the serum sample of patients with LSCC，especially in samples from individuals with cervical lymphatic metastasis.（3）High positive detection rates were obtained through independent testing of anti-survivin，anti-p53 and anti-c-myc antibodies;the combined positive rate of all three antibodies was inferior to that of anti-survivin antibody but superior to that of anti-p53 and anti-c-myc antibodies. Conclusions：This indicates that anti-survivin antibody may be a biomarker of LSCC. Combined testing of three different tumor markers may be valuable for monitoring of the surgery efficacy.