Comparison on three transfection methods for ZsGreen gene expression mediated by adeno-associated virus in the myocardium of rats

TAN Wenpeng; YANG Kan; LI Anying; YANG Qiong

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Comparison on three transfection methods for ZsGreen gene expression mediated by adeno-associated virus in the myocardium of rats

更新时间：2023-12-18

- Comparison on three transfection methods for ZsGreen gene expression mediated by adeno-associated virus in the myocardium of rats
- Journal of Chongqing Medical University Vol. 37, Issue 6, Pages: 522-524(2012)
- 作者机构：
  
  中南大学湘雅三医院心内科,长沙,410013
- 作者简介：
- 基金信息：
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- Published：2012，
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TAN Wenpeng, YANG Kan, LI Anying, et al. Comparison on three transfection methods for ZsGreen gene expression mediated by adeno-associated virus in the myocardium of rats. [J]. Journal of Chongqing Medical University 37(6):522-524(2012)
DOI：

TAN Wenpeng, YANG Kan, LI Anying, et al. Comparison on three transfection methods for ZsGreen gene expression mediated by adeno-associated virus in the myocardium of rats. [J]. Journal of Chongqing Medical University 37(6):522-524(2012) DOI：

摘要

目的：采用腺相关病毒（Adeno-associated virus，ＡＡＶ）-9为载体，比较３种转染方法对ZsGreen基因在大鼠心肌组织表达的影响。方法：30只雄性SD大鼠随机分为尾静脉（Tail vein，TV）注射组、心肌（Intramyocardium，IM）注射组、冠脉（Intra－coronary，IC）灌注组，采用相应方法在体转染携带报告基因ZsGreen的腺相关病毒-9型（Adeno-associated virus 9-ZsGreen，AAV9-ZsGreen）。2周后荧光显微镜下观察绿色荧光在大鼠ＩＭ组织的表达情况。结果：TV组ＩＭ有少量绿色荧光分布；IM组绿色荧光表达主要局限在ＩＭ注射位点，注射点周边有少量分布；IC组大量绿色荧光在ＩＭ广泛均匀表达。IM及IC组荧光强度均显著高于TV组（P＜0.01），IC组荧光强度高于IM组（P＜0.01）。结论：IC灌注法可使ＡＡＶ介导的外源基因在ＩＭ组织高效、广泛、均匀表达，优于TV注射法及ＩＭ注射法。

Abstract

Objective：To compare the effect of three transfection methods on ZsGreen gene expression in rats myocardium mediated by adeno-associated virus 9（AAV9）. Methods：Totally 30 male SD rats were divided into tail vein（TV） injection group，intramyocardium（IM） injection group and intracoronary（IC） infusion

关键词

转染心肌腺相关病毒基因治疗

Keywords

转染心肌腺相关病毒基因治疗

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