Preparation of targeted high molecular polymer microbubble encapsuling quantum dots and cell experiment study in vitro

HAO Lan; WANG Zhigang; LUO Jie; ZHANG Qunxia; ZHENG Yuanyi

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Preparation of targeted high molecular polymer microbubble encapsuling quantum dots and cell experiment study in vitro

更新时间：2023-12-18

- Preparation of targeted high molecular polymer microbubble encapsuling quantum dots and cell experiment study in vitro
- Journal of Chongqing Medical University Vol. 36, Issue 1, Pages: 24-27(2013)
- 作者机构：
  
  重庆医科大学超声影像学研究所,重庆,400010
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2013，
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HAO Lan, WANG Zhigang, LUO Jie, et al. Preparation of targeted high molecular polymer microbubble encapsuling quantum dots and cell experiment study in vitro. [J]. Journal of Chongqing Medical University 36(1):24-27(2013)
DOI：

HAO Lan, WANG Zhigang, LUO Jie, et al. Preparation of targeted high molecular polymer microbubble encapsuling quantum dots and cell experiment study in vitro. [J]. Journal of Chongqing Medical University 36(1):24-27(2013) DOI：

摘要

目的：制备一种量子点（quantum dots，QDs）标记单抗赫赛汀修饰的靶向高分子微泡 MBQDs/PLGA/Her，并考察其在人乳腺癌细胞MCF-7靶向性实验中的应用。方法：双乳化法（W1/O/W2）联合共价偶联法（EDC/NHS）制备MBQDs/PLGA/Her，TEM表征QDs装载标记，MTT法比较细胞毒性，傅里叶变换红外光谱（Fourier transform infrared spectrum，FT-IR）检测MBQDs/PLGA/Her与抗体的连接情况，荧光显微镜观察QDs荧光标记性能。结果：MBQDs/PLGA/Her平均粒径为（584.7±20.2） nm，TEM显示QDs在聚乳酸-羟基乙酰共聚物（poly lactic-glycolic acid，PLGA）微粒包埋，FT-IR表明赫赛汀氨基与PLGA羧基的共价偶联。MBQDs/PLGA/Her细胞毒性小于QDs试剂（P<0.05）。细胞识别实验表明QDs标记的红色微泡MBQDs/PLGA/Her紧密黏附在人乳腺癌细胞MCF-7周围。结论：成功制备MBQDs/PLGA/Her，QDs包裹在高分子微气泡核内既改善其细胞毒性且保持QDs荧光标记性能。

Abstract

Objective：To prepare a high molecular polymer microbubble MBQDs/PLGA/Her marked by quantum dots（QDs） and modified by single antibody Herceptin and to study its application in targeted experiment of human breast cancer cells MCF-7. Methods：MBQDs/PLGA/Her targeted microbubble was produced by the double emulsion method（W1/O/W2） and covalent coupling technique（EDC/NHS）. Its physi－cal properties（loading mark of QDs，cytotoxicity，connection of MBQDs/PLGA /Her to antibody，fluorescent marking performance of QDs） were determined by methods of TEM，MTT，Fourier transform infrared spectrum（FT-IR） and fluorescence microscope respectively. Results：MBQDs/PLGA/Her average diameter was （584.7±20.2） nm. TEM imaging showed that QDs was loaded in recombinant human tissue-type plasminogen activator poly lactic-glycolic acid（PLGA） particles and FT-IR spectrogram confirmed covalent coupling between Her－ceptin’s amino and PLGA’s carboxyl. Cell toxicity of MBQDs/PLGA/Her was less than that of QDs reagents（P<0.05）. Red microbubble MBQDs/PLGA/Her marked by QDs was connected round human breast cancer cells MCF-7. Conclusions：MBQDs/PLGA/Her microbubble is prepared successfully. Targeted microbubble cell toxicity is improved and QDs fluorescent marking performance is kept by loading QDs into polymer microbubble.

关键词

量子点高分子微气泡抗体标记

Keywords

quantum dotshigh molecular polymer microbubbleantibodylabeling

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