Objective：To explore the effects of celecoxib，ibuprofen and indomethcin on expressions of interleukin-1β（IL-1β），tumor necrosis factor-α（TNF-α） and interleukin-1α（IL-1α） in osteoarthritic chondrocytes of Wistar rats. Methods：One hundred and thirty Wistar rats were randomly divided into 4 groups：celecoxib（CE） group，ibuprofen（IBP） group，indomethacin group（IN） and normal saline（NS） group. Osteoarthritis（OA） was induced by the excision of the left achilles tendon. In the 3rd，6th and 9th month of the treatment after surgically induced OA，the articular cartilage was observed with immunohischemical method to estimate the expres－sions of IL-1β，TNF-α and IL-1α in osteoarthritic chondrocytes of 4 groups. Results：①In the 3rd month，CE exerted no remarkable effects on IL-1β and TNF-α but suppressed expressions of IL-1α；IBP promoted expressions of IL-1β and TNF-α but exerted no re－markable effects on IL-1α；IN mildly promoted expressions of IL-1β，enhanced expressions of TNF-α but exerted no remarkable ef－fects on IL-1α. ②In the 6th month，CE exerted no remarkable effects on IL-1β and mildly promoted TNF-α and IL-1α expressions；IBP promoted expressions of IL-1β（mildly） and TNF-α but mildly suppressed expressions of IL-1α；IN suppressed expressions of IL-1β（mildly），IL-1α and TNF-α（mildly）. ③In the 9th month，CE suppressed expressions of IL-1β（significantly），TNF-α and IL-1α；IBP suppressed expressions of IL-1β，TNF-α and IL-1α（mildly）. Conclusions：During the long-term use of three kinds of nons－teroidal anti-inflammatory drugs in OA，CE can inhibit the expression of IL-1β，TNF-α and IL-1α in osteoarthritic chondrocytes and consequently mitigate the development of OA. Though，IBP and IN exert different influences on expressions of IL-1β，TNF-α and IL-1α during different periods，the overall suppression effects are not obvious.