Establishment of one-lung ventilation induced acute lung injury rabbit model by combining right mainstem bronchial intubation with parasternal fenestration

LIU Rui; LI Yanhua; GUO Xin; YANG Yong; WANG Dianhua

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Establishment of one-lung ventilation induced acute lung injury rabbit model by combining right mainstem bronchial intubation with parasternal fenestration

更新时间：2023-12-18

- Establishment of one-lung ventilation induced acute lung injury rabbit model by combining right mainstem bronchial intubation with parasternal fenestration
- Journal of Chongqing Medical University Vol. 36, Issue 6, Pages: 653-656(2013)
- 作者机构：
  
  1. 昆明医科大学附属昆华医院麻醉科,昆明,650032
  2. 昆明医科大学医学机能实验中心,昆明,650031
  3. 昆明医科大学药学院暨云南省天然药物药理重点实验室,昆明,650031
- 作者简介：
- 基金信息：
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  CLC：
- Published：2013，
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LIU Rui, LI Yanhua, GUO Xin, et al. Establishment of one-lung ventilation induced acute lung injury rabbit model by combining right mainstem bronchial intubation with parasternal fenestration. [J]. Journal of Chongqing Medical University 36(6):653-656(2013)
DOI：

LIU Rui, LI Yanhua, GUO Xin, et al. Establishment of one-lung ventilation induced acute lung injury rabbit model by combining right mainstem bronchial intubation with parasternal fenestration. [J]. Journal of Chongqing Medical University 36(6):653-656(2013) DOI：

摘要

目的：运用右主支气管内插管合并胸骨旁开窗术构建单肺通气（one-lung ventilation，OLV）致兔急性肺损伤（acute lung injury，ALI）动物模型。方法：24只健康日本大耳白兔随机分为以下4组（n=6）：假手术组（S组）；双肺通气（two-lung ventilation，TLV）组（T组）；TLV加胸骨旁开窗术（parasternal fenestration，ＰＦ）组（TＰF组）和OLV组（O组）。用支气管肺泡灌洗液（bron－choalveolar lavage fluid，BALF）中中性粒细胞（polymorphonuclear leukocyte，PMN）计数，肺组织髓过氧化物酶（myeloperoxidase，MPO）含量，肺湿/干（wet/dry，W/D）比值和肺组织形态学评分评价肺损伤严重程度。结果：与S组相比，O组、T组与TPF组动物BALF中PMN计数，肺组织MPO活性，肺W/D比值和肺组织形态学评分均明显增高（P<0.05）；TPF组和T组动物间上述各项指标差异无统计学意义，但均低于O组（P<0.05）。结论：右主支气管内插管合并双侧ＰＦ是一种用于构建OLV致兔ALI的可靠方法。

Abstract

Objective：To build one-lung ventilation（OLV) induced acute lung injury（ALI） rabbit model by combining right mainstem bronchial intubation with parasternal fenestration. Methods：Totally 24 healthy Japanese white rabbits were randomized into 4 groups （n=6）：sham operated group（S group），two-lung ventilation（TLV） group（T group），TLV with parasternal fenestration group（TＰF group） and one-lung ventilation group（O group）. Lung injury was judged by polymorphonuclear leukocyte（PMN） count in bron－choalveolar lavage fluid（BALF），myeloperoxidase（MPO） content in pulmonary tissues，lung wet/dry（W/D） ratio and pulmonary histo－logical score. Results：Compared with those in S group，all indicators mentioned above were increased significantly in O group，T group and TF group（P<0.05）. No difference was observed between T group and TF group，but all indicators in the two groups were lower than those in O group（P<0.05）. Conclusions：Combining right mainstem bronchial intubation with parasternal fenestration is a simple and reliable method to build rabbit model of OLV induced ALI.

关键词

单肺通气急性肺损伤动物模型

Keywords

one-lung ventilationacute lung injuryanimal model

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