Determination of erbascoside and resveratrol in root of polygonum cuspidatum by high performance liquid chromatography

WU Qing; LUO Xianqin; HU Rong

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Determination of erbascoside and resveratrol in root of polygonum cuspidatum by high performance liquid chromatography

更新时间：2023-12-18

- Determination of erbascoside and resveratrol in root of polygonum cuspidatum by high performance liquid chromatography
- Journal of Chongqing Medical University Vol. 36, Issue 7, Pages: 773-775(2013)
- 作者机构：
  
  1. 重庆医科大学附属儿童医院药剂科,重庆,400014
  2. 重庆市中药研究院药理毒理研究所,重庆,400065
  3. 重庆市中药研究院制剂所,重庆,400013
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2013，
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WU Qing, LUO Xianqin, HU Rong. Determination of erbascoside and resveratrol in root of polygonum cuspidatum by high performance liquid chromatography. [J]. Journal of Chongqing Medical University 36(7):773-775(2013)
DOI：

WU Qing, LUO Xianqin, HU Rong. Determination of erbascoside and resveratrol in root of polygonum cuspidatum by high performance liquid chromatography. [J]. Journal of Chongqing Medical University 36(7):773-775(2013) DOI：

摘要

目的：建立虎杖提取物中白藜芦醇的含量测定方法。方法：采用高效液相色谱法测定虎杖提取物中白藜芦醇的含量。色谱条件：流动相：乙腈-水（30∶70）；流速：1.0 ml/min；检测波长：306 nm；柱温：室温；进样量：10 μl。结果：白藜芦醇进样量在0.3～9.6 μg/ml范围内，线性关系良好。结论：本法简便实用、灵敏度高、重现性好，可用于虎杖提取物中白藜芦醇的含量测定。

Abstract

Objective：To establish reserved-phase high performance liquid chromatography（HPLC） method for the determination of resveratrol content in root of polygonum cuspidatum. Methods：HPLC was carried out to detect resveratrol content in root of polygonum cuspidatum. Chromatographic conditions：mobile phase consisting of acetonitrile-water（30∶70） at flow rate of 1.0 ml/min；detection wave length of 306 nm；column temperature at room temperature；sample size of 10 μl. Results：Calibration curves were linear within the range of 0.3~9.6 μg/ml. Conclusions：The method is simple，accurate，reproducible，precise and can be used in the determination of resveratrol content in polygonum cuspidatum extract.

关键词

虎杖提取物白藜芦醇高效液相色谱法

Keywords

polygonum cuspidatum extractresveratrolhigh performance liquid chromatography

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