Effects of interleukin-10 modification on in vitro immunosuppression of bone marrow mesenchymal stem cells

FANG Zhenqiang; DAI Wei; JIA Weisheng; HE Fan; SHEN Chongxing; LIU Yongliang; ZHOU Feng; YE Gang

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Effects of interleukin-10 modification on in vitro immunosuppression of bone marrow mesenchymal stem cells

更新时间：2023-12-18

- Effects of interleukin-10 modification on in vitro immunosuppression of bone marrow mesenchymal stem cells
- Journal of Chongqing Medical University Vol. 36, Issue 11, Pages: 1300-1302(2013)
- 作者机构：
  
  1. 第三军医大学新桥医院泌尿外科，重庆４０００３７
  2. 重庆石油医院内科,重庆,400037
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2013，
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FANG Zhenqiang, DAI Wei, JIA Weisheng, et al. Effects of interleukin-10 modification on in vitro immunosuppression of bone marrow mesenchymal stem cells. [J]. Journal of Chongqing Medical University 36(11):1300-1302(2013)
DOI：

FANG Zhenqiang, DAI Wei, JIA Weisheng, et al. Effects of interleukin-10 modification on in vitro immunosuppression of bone marrow mesenchymal stem cells. [J]. Journal of Chongqing Medical University 36(11):1300-1302(2013) DOI：

摘要

目的：研究白细胞介素-10（interleukin-10，IL-10）基因修饰的骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMMSCs）体外免疫效应。方法：RT-PCR克隆大鼠IL-10基因并构建逆转录病毒pLNCX-IL-10，转染大鼠BMMSCs；流式细胞仪检测IL-10基因修饰的BMMSCs（BMMSCsIL-10）体外对T淋巴细胞亚群的影响，CCK-8法检测BMMSCsIL-10及其培养上清液对体外混合淋巴细胞增殖的影响。结果：BMMSCsIL-10能明显减少CD4+ T，降低CD4+/CD8+比值，比BMMSCs作用明显（P=0.000）；BMMSCsIL-10及其培养上清液比BMMSCs更能明显抑制T淋巴细胞增殖反应（P=0.000）。结论：IL-10基因修饰能增强BMMSCs的体外免疫抑制作用。

Abstract

Objective：To study the in vitro immunosuppression of interleukin-10（IL-10） modified bone marrow mesenchymal stem cells（BMMSCsIL-10）. Methods：IL-10 was cloned by RT-PCR and was used to construct pLNCX-IL-10. The constructed retrovirus was transfected into rat BMMSCs. T lymphocyte subsets in vitro influenced by BMMSCsIL-10 were detected by flow cytometry. Effects of BMMSCsIL-10 and its culture supernatant on in vitro mixed heterologous T lymphocyte proliferation were detected by CCK-8. Results：BMMSCsIL-10 significantly reduced CD4+ T and lowered the ratio of CD4+/CD8+，more intense than BMMSCs（P=0.000）. BMMSCsIL-10 and its culture supernatant significantly inhibited T lymphocyte proliferation，more intense than BMMSCs（P=0.000）. Conclusions：IL-10 modification can enhance the in vitro immunosuppressive effect of BMMSCs.

关键词

骨髓间充质干细胞白细胞介素-10免疫抑制

Keywords

bone marrow mesenchymal stem cellsinterleukin-10immunosuppression

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