Objective：To explore the effects of Shenfu injection pretreatment on intestinal epithelium of endotoxemia rats. Methods：Forty-eight adult male rats were randomly divided into endotoxemia model group（group M，n=24） and Shenfu injection pretreatment group（group S，n=24）. Rats in group M and group S were further divided into 2 h，6 h，12 h subgroups and each subgroup had eight rats. A single caudal venous injection of lipopolysaccharide（10 mg/kg） was used to induce endotoxemia model. Thirty minutes before endotoxemia model was induced，rats in group M and group S respectively intraperitoneally received saline（5 ml/kg） and Shenfu in－jection（5 ml/kg） and samples were respectively obtained at each time point. Another eight rats did not do any treatment were taken as control group（group C，n=8） and samples was collected directly. Blood was collected through abdominal aorta to measure intestinal fatty acid binding protein（i-FABP） and diamine oxidase（DAO） in serum by ELISA. Distal ileum was collected；levels of tumor necro－sis factor-α（TNF-?琢） and interleukin-6（IL-6） in intestinal mucosa were detected by ELISA；morphological changes were observed by HE for staining and pathological scores were evaluated according to Chiu’s score. Results：I-FABP and DAO levels in blood plas－ma，TNF-?琢 and IL-6 levels in intestinal mucosa and Chiu’s scores in group M were all significantly increased at each time point compared with those in group C（P<0.05）. At 2 h after endotoxemia model establishment，i-FABP，DAO，TNF-?琢 and IL-6 levels as well as Chiu’s scores in group S were lower than those in group M（P<0.05），meanwhile，TNF-?琢 and IL-6 levels in group S were low－er than those in group M（P<0.05）. Conclusions：Shenfu injection pretreatment on endotoxemia rats could attenuate inflammatory re－sponse，decrease serum i-FABP and DAO levels，lower pathological scores and alleviate the injury of intestinal epithelium at early stage.