Objective：To evaluate the effect of implementing targeted nosocomial infection monitoring and intervention in intensive care unit（ICU）. Methods：Nosocomial infection condition of hospitalized patients，risk factors and nosocomial infection changing trend in comprehensive ICU from 2011 to 2012 were mastered by targeted nosocomial infection monitoring and intervention. Results：Infec－tion rates of ICU in 2011 and 2012 were 20.9% and 16.7 % and case infection rates were 22.7% and 18.0%. After average severity of illness score adjustion，adjusted daily infection rates were 5.7 ‰ and 4.4 ‰（ χ2=0.321，P=0.865） and adjusted daily infected cases rates were 5.9 ‰ and 4.7 ‰（ χ2=0.560，P=0.454）. Infection rates of ventilator-associated pneumonia in 2011 and 2012 were 29.0 ‰ and 20.1 ‰. Infection rates of central venous catheter-related bloodstream were 1.8 ‰ and 1.2 ‰. Infection rates of catheter related urinary tract were 1.3 ‰ and 0.7 ‰. In 2012，the overall detection rate of multi-drug resistant bacteria was in downward trend and detection rate of methicillin-resistant staphylococcus aureus was decreased after taking interventions，with statistically significant dif－ferences（ χ2=9.05，P=0.029）. Conclusions：Implementation of targeted monitoring and infection control measures can effectively re－duce the incidence of nosocomial infection ICU.