Expression and clinical significances of Has-miRNA-96 in tissues of cervical cancer
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Expression and clinical significances of Has-miRNA-96 in tissues of cervical cancer
Journal of Chongqing Medical UniversityVol. 38, Issue 3, Pages: 405-408(2014)
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广州军区广州总医院妇产科,广州,510010
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Published:2014,
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Zhou Wen, Hao Chenjun, Guan Ting. Expression and clinical significances of Has-miRNA-96 in tissues of cervical cancer. [J]. Journal of Chongqing Medical University 38(3):405-408(2014)
DOI:
Zhou Wen, Hao Chenjun, Guan Ting. Expression and clinical significances of Has-miRNA-96 in tissues of cervical cancer. [J]. Journal of Chongqing Medical University 38(3):405-408(2014)DOI:
Expression and clinical significances of Has-miRNA-96 in tissues of cervical cancer
Objective:To evaluate has-miRNA-96(miR-96) expression in cervical cancer tissues and their association with the clini-copathological features. Methods:Expression of miR-96 in 52 cases of cervical cancer tissues and 28 cases of normal tissues was examined by stem-loop real-time PCR. Correlations between expression of miR-96 and related clinicopathologic features of cervical cancer(age,pathologic pattern,histological grade,depths of interstitial infiltration,lymph node metastasis,clinical stage) were further analyzed. Results:Relative expression of miR-96 was significantly higher in cancer tissues(127.045±235.424) than in normal tis-sues(0.995±0.236)(P=0.000). Expression of miR-96 was significantly higher in middle and low differentiated carcinoma(137.778±249.981) than in high differentiated carcinoma(75.766±147.237),with statistical differences(P=0.005). Expression of miR-96 was significantly higher in cervical adenocarcinoma(196.213±172.519) than in squamous cell carcinomas(110.576±246.910)(P=0.004). Expression of miR-96 at Ⅰsatge,Ⅱstage and Ⅲ-Ⅳ stage was (22.614±25.828),(122.493±78.043) and (672.902±476.169) respectively;expression at Ⅲ-Ⅳ stage was significantly higher than that at Ⅰstage and Ⅱstage(P=0.000,P=0.001) and expression at Ⅱstage was significantly higher than that at Ⅰstage(P=0.000). Expression of miR-96 was significantly higher in those with lymphatic metastasis(142.537±104.673) than those without(19.787±26.204)(P=0.000). Patients with invasive depth<1/2 in-terstitial substance(27.449±49.728) showed lower level of miR-96 than those with invasive depth ≥1/2 interstitial substance(75.323±94.822)(P=0.025). Expression of miR-96 was not correlated with age(P=0.385). Conclusions:Aberrant expression of miR-96 sug-gests it might play a role as an oncogene in the tumorigenesis and development of cervical carcinoma and it possibly correlates with progression and prognosis of cervical cancer.
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