Objective：To explore the interaction between olfaetomedin 4（OLFM4） mRNA and nucleolin protein and to discuss the regulatory effect of nucleolin protein on the transcription level of OLFM4. Methods：RNA chromatin chromatin immunoprecipitation assay（RNA chip） combined with RT-PCR was performed to identify whether OLFM4 interacted with nucleolin and the effects of nucleolin on the OLFM4 were detected by dual-luciferase reporter assay. Results：OLFM4 directly interacted with the nucleolin in SGC-7901 cells. Relative luciferase activity of over expression of nucleolin plasmid was about 1.15 times of the control plasmid；relative luciferase activity of knockdown of nucleolin plasmid was about 0.8 times of the control plasmid；nucleolin had positive moderating effect on OLFM4. Conclusions：OLFM4 directly interacts with the nucleolin and nucleolin stabilizes the OLFM4 mRNA by increasing OLFM4 transcription and extending OLFM4 mRNA half-life.