Objective：To investigate the effect of chronic cerebral hypoperfusion on astrocytes in mice brain. Methods：Three months old C57 mice were subjected to chronic cerebral hypoperfusion with 2 vessels occlusion or sham operation. Anilin blue staining was used to prove the effectiveness of the model. Pathological changes of astrocytes were examined by immunohistochemicalstaining（IHC） of glial fibrillary acidic protein（GFAP）. Western blot was employed to test the levels of GFAP in brain. Results：After the Anilin blue perfusion，cerebrum of model 0 h group was not dyed while that of sham control group was dyed dark blue. After chronic cerebral hypoperfusion，astrocytes were proliferated and manifested as cell quantity increase，cellular hypertrophy and processes. Two months after model establishment，number of GFAP positive cells was increased，dying was deepened and integrated optical density was enhanced compared with those in sham control group，with statistical differences（P<0.05）. Intracerebral GFAP expression in model group was increased significantly compared with that in sham control group，with statistical differences（P<0.05）. Conclusions：Astro－cytes are consistently proliferated after chronic cerebral hypoperfusion，which play an important role in irreversible pathological changes after cerebral ischemia.