Objective：To research the effect of gypenosides－containing serum on the expression of proto-oncogene c-Fos and c-Jun in ultraviolet irradiated cells. Methods：Blank serum and gypenosides-containing serum were made. Human immortal skin keratinocytes （HaCaT） were divided into 4 groups（A-D），10 bottles in each group. Human skin fibroblast（HSF） were divided into 6 groups（Ⅰ-Ⅵ），10 bottles in each group. B，C，D groups of irradiated HaCaT and Ⅱ，Ⅲ，Ⅳ，Ⅴ，Ⅵ groups of HSF underwent UVB and UVA radiation. Supernatant of 4 groups HaCaT was put into HSF Ⅲ-Ⅵ groups in turn. Six groups for measurement were made：blank control group（Ⅰgroup），UVA model group（Ⅱgroup），HaCaT supernatant A group（Ⅲ group），HaCaT supernatant B group（Ⅳ group），HaCaT supernatant C group（Ⅴgroup），HaCaT supernatant D group（Ⅵ group）. Expression levels of c-Fos and c-Jun mRNA and protein in each group were measured by RT-PCR and Western blot. Results：c-Fos and c-Jun mRNA and protein expression was significantly higher in groupⅡ than in groupⅠ（all P =0.000）. c-Fos and c-Jun mRNA and protein expression was significantly higher in group Ⅳ than in group Ⅱ（Pc-Fos mRNA＝0.016，Pc-Fos protein＝0.001，Pc-Jun mRNA＝0.005，Pc-Jun protein＝0.000）. Changes in group Ⅲ were not significant. c-Fos and c-Jun mRNA and protein expression was significantly lower in group Ⅵ than in group Ⅳ（all P =0.000）. Changes in group Ⅴ were not significant（Pc-Fos mRNA＝0.081，Pc-Fos protein＝0.088，Pc-Jun mRNA＝0.109，Pc-Jun protein＝0.026）. Conclusions：The UVB irradiated HaCaT cell secrete cytokine to increase c-Fos and c-Jun mRNA and protein expression in UVA irradiated cell HSF. Gypenosides can inhibit c-Fos and c-Jun mRNA and protein expression of UVA irradiated HSF cells which are affected by UVB irradiated HaCaT supernatant.