Objective：To analyze the distribution and drug susceptibility of bacteria isolated from cerebrospinal fluid（CSF） in patients who developed nosocomial meningitis following neurosurgical operation and to provide evidences for clinical treatment of meningitis.Methods：Clinical data of pathogens and their drug susceptibility to antibiotics from CSF were collected from January 2008 to Decem－ber 2012. Distribution and drug susceptibility of bacteria were analyzed retrospectively. Results：Positive rate of pathogens was 22.35（114/510） from 510 CSF specimens. There were 114 strains of pathogens including 58（50.88%） G+ bacteria and 56（49.12%） G- bacteria. The most frequently isolated bacteria was coagulase-negative staphylococci（28.95%），followed by acinetobacter baumannii（16.67%），enterococcus spp.（9.65%），pseudomonas aeruginosa（9.65%），klebsiella pneumoniae（6.14%），staphylococcus aureus. The active compounds against G+ bacteria were vancomycin（100%），linezolid（95.92%），piperacillin-tazobactam（88.89%）. The active compounds against G- bacteria were imipenem（66.04%），followed by piperacillin-tazobactam（59.46%），amikacin（55.10%）. Super-infection in bacterial meningitis was observed in 8 patients. Conclusions：G+ bacteria are more common in neurosurgical patients，es－pecially coagulase-negative staphylococci，however，G- pathogens has increased recently，especially acinetobacter baumannii，therefore nosocomial meningitis pathogens should be considered in the choice of empirical antibiotic treatments.