Objective：To access the influence of hematuria on the performance of urine cytology（CYT） and fluorescence in situ hy－bridization（FISH） in the detection of bladder carcinoma（BC）. Methods：From January 2009 to June 2013，a total of 164 patients sus－picious of BC underwent tests of CYT and FISH followed by upper urinary tract imaging and cystoscopy. Transurethral biopsy and/or resection were performed on suspicious lesions. The hematuria was determined by urine microscopy. Effect of hematuria on false pos－itive and negative rates of patients with and without BC was compared. Results：For patients with hematuria，false positive rates of CYT were 14.3% and 20.9%（χ2=0.645，P=0.422） and false negative rates of CYT were 21.4% and 39.1%（χ2=2.618，P=0.106）；false positive rates of FISH were 19.0% and 20.9%（χ2=0.047，P=0.828） and false negative rates of FISH were 30.3% and 30.4%（χ2=0.002，P=0.967） all without statistical difference. Conclusion：Hematuria does not influence the performance of CYT and FISH.