Objective：To establish a reversed-phase high-performance liquid chromatographic method to determine the content of naringin in nanofibers. Methods：The test film，nanofiber film containing naringin，was prepared using electrospinning equipment. The determination was performed on Agilent C18 chromatographic column（4.6 mm×150 mm，5 μm）. The acetonitrile-phosphoric water （pH 2.8） （23∶77） was used and the detecting wavelength was 282 nm. The column temperature was 30 ℃. The sampling inlet vol－ume was 20 μl. Results：Pure PCL nanofiber，having good specificity exerted no interfering effect on naringin determination. The low－est detection limit of naringin S/N=3 was 3.4 ng/ml. The limit of quantitation S/N=10 was 10.2 ng/ml. Good linear dependence（corre－lation coefficient r=0.999 99） was detected when the concentration of naringin was within the range of 51-1 836 μg/ml. This method had good precision and repeatability both within and between days. The average recovery of naringin was 99.51% and the relative standard deviation was 0.66%. The test solution was stable within 24 h. Conclusion：Naringin nanofiber film can be produced using electrospinning technique which is a nano film with medicine loaded for bone regenerative. This study provides an easy，accuracy，rapid way for the quantitative determination of naringin in nanofibers and solid basis for the evaluation of the optimum content of naringin in nanofibers.