Objectives：To study the effect of appling clinical nursing path in the third stage of chemotherapy for pediatric neuroblas－toma（NB） based on evidence-based nursing. Methods：Totally 120 children with NB who underwent chemotherapy neuroblastoma were randomly divided into experimental group（n=60） and control group（n=60）. Experimental group intended to find evidences from nursing assessment，nursing plan，nursing measures and health education and clinical nursing path table was made and used in this study. Patients in control group were nursed according to conventional nursing methods. Results：Patients in experimental group were more satisfied with hospitalization days and hospitalization expenses than those in control group. Patients in experiment group under－stood health education better than those in control group（P<0.01）. Incidences of chemotherapy side reactions were significantly re－duced in experiment group than in control group（P<0.05）. Conclusion：Clinical nursing path based on evidence-based nursing can effectively reduce the hospitalization days，hospitalization expenses and side effects of chemotherapy. For children and their families， the degree of understanding health education knowledge and the satisfaction of nursing service can be improved obviously.