Influence of docetaxel-loaded lipid microbubbles combined with ultrasound targeted microbubbles destruction on proliferation and apoptosis of hepatocellular carcinoma cell

Kang Juan; Wu Xiaoling; Zhang Dazhi; Zhou Zhi; Hu Peng; Wang Zhigang

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Influence of docetaxel-loaded lipid microbubbles combined with ultrasound targeted microbubbles destruction on proliferation and apoptosis of hepatocellular carcinoma cell

更新时间：2023-12-18

- Influence of docetaxel-loaded lipid microbubbles combined with ultrasound targeted microbubbles destruction on proliferation and apoptosis of hepatocellular carcinoma cell
- Journal of Chongqing Medical University Vol. 38, Issue 12, Pages: 1698-1702(2014)
- 作者机构：
  
  1. 重庆医科大学附属第二医院肝病治疗研究中心,重庆,400010
  2. 重庆医科大学附属第二医院消化内科,重庆,400010
  3. 重庆医科大学超声影像学研究所,重庆,400010
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2014，
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Kang Juan, Wu Xiaoling, Zhang Dazhi, et al. Influence of docetaxel-loaded lipid microbubbles combined with ultrasound targeted microbubbles destruction on proliferation and apoptosis of hepatocellular carcinoma cell. [J]. Journal of Chongqing Medical University 38(12):1698-1702(2014)
DOI：

Kang Juan, Wu Xiaoling, Zhang Dazhi, et al. Influence of docetaxel-loaded lipid microbubbles combined with ultrasound targeted microbubbles destruction on proliferation and apoptosis of hepatocellular carcinoma cell. [J]. Journal of Chongqing Medical University 38(12):1698-1702(2014) DOI：

摘要

目的：初步研究载多西紫杉醇脂质微泡（docetaxel-loaded lipid microbubbles，LDLM）联合超声靶向微泡破裂（ultrasound targeted microbubbles destruction，UTMD）对人SMMC-7721肝癌细胞增殖和凋亡的影响。方法：体外培养人SMMC-7721细胞，确定适宜的多西紫杉醇浓度，细胞分为6组，比较各组的细胞增殖抑制率、超微结构改变、凋亡率和细胞周期分布。结果：载药微泡+超声组的细胞增殖抑制率明显高于其他组，与其他各组相比差异有统计学意义（P=0.000）；电镜下观察载药微泡+超声组的凋亡细胞最多；流式细胞仪检测结果显示载药微泡+超声组的细胞凋亡率最高（P=0.000），LDLM+US组G0/G1期细胞比例明显减少，为（0.79±0.27）%（P=0.000），G2/M期细胞比例升高最明显，为（90.54±0.48）%（P=0.000）。结论：LDLM联合UTMD可抑制人SMMC-7721细胞的增殖、促进其凋亡，为后续进一步从蛋白、基因层面探索其作用的机制奠定了重要基础。

Abstract

Objective：To study the influence of docetaxel-loaded lipid microbubbles（LDLM） combined with ultrasound targeted mi－crobubbles destruction（UTMD） on proliferation and apoptosis of SMMC-7721 cells. Methods：Suitable concentration of docetaxel was chosen. SMMC-7721 cells were cultured in vitro and divided into 6 groups. Proliferating inhibition rate，ultrastructural change，apop－totic rate and cell cycle distribution were compared among all groups. Results：The proliferating inhibition rate and apoptotic rate of LDLM+US group were the highest compared with that of other groups（P=0.000，P=0.000）. Great amount of apoptotic cells were observed in LDLM+US group by electron microscope and flow cytometry. Proportion of cells at G0/G1 phase was decreased obviously（0.79±0.27）%（P=0.000） while that at G2/M phase was obviously increased（90.54±0.48）%（P=0.000）. Conclusion：LDLM combined with UTMD could inhibit the proliferation and promote the apoptosis of SMMC-7721 cells，which provide evidence for the further study of its mechanisms in protein and gene level.

关键词

超声微泡多西紫杉醇人肝癌细胞系载药微泡人SMMC-7721细胞

Keywords

ultrasound microbubbledocetaxelhuman hepatocellular carcinoma cell linedrug-loaded microbubbleSMMC-7721 cell

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