Effects of high intensity focused ultrasound on lysozyme structure

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Effects of high intensity focused ultrasound on lysozyme structure

更新时间：2023-12-18

- Effects of high intensity focused ultrasound on lysozyme structure
- Journal of Chongqing Medical University Vol. 40, Issue 2, Pages: 252-255(2015)
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Effects of high intensity focused ultrasound on lysozyme structure. [J]. Journal of Chongqing Medical University 40(2):252-255(2015)
DOI：

Effects of high intensity focused ultrasound on lysozyme structure. [J]. Journal of Chongqing Medical University 40(2):252-255(2015) DOI：

摘要

目的：探讨高强度聚焦超声（high intensity focused ultrasound，HIFU）对一种蛋白质—溶菌酶结构的影响。方法：使用频率0.94 MHz、声强3.25×104 W/cm2、脉冲重复频率4 Hz、占空比10%的脉冲高强度聚焦超声辐照溶菌酶溶液10 s。利用圆二色光谱（CD光谱）、荧光光谱、核磁共振氢谱（1H nuclear magnetic resonance，1H-NMR）和聚丙烯酰胺凝胶电泳（sodium dodecyl sulfate-polyacrylamide gel，SDS-PAGE）方法，分别研究高强度聚焦超声对溶菌酶二级、三级和四级结构的影响。结果：高强度聚焦超声处理后，CD光谱无明显变化，表明高强度聚焦超声对溶菌酶二级结构影响不大；而荧光强度减弱，说明溶菌酶色氨酸周围的疏水结构发生了变化；SDS-PAGE结果显示，经高强度聚焦超声辐照后溶菌酶出现支链降解；分析 1H-NMR发现，辐照后溶菌酶色氨酸发生了化学位移。结论：高强度聚焦超声可以改变溶菌酶的高级结构。

Abstract

Objective：To investigate the effect of high intensity focused ultrasound（HIFU） on the lysozyme molecular structure. Meth－ods：The lysozyme solution was exposed at HIFU with pulse frequency of 0.94 MHz，sound intensity of ３.25×104 W/cm2，pulse repetition period of 4 Hz and duty cycle of 10% for 10 s. The effect of HIFU on the lysozyme molecular structure was studied using CD spectra，fluorescence spectrum，1H nuclear magnetic resonance（1H-NMR） and sodium dodecyl sulfate-polyacrylamide gel（SDS-PAGE） method. Results：The Result showed that after HIFU treatment，there was no obvious change in CD spectrum，indicating that HIFU might cause little effect on lysozyme lower structure. Whereas a weaken fluorescence intensity appeared indicating lysozyme conformation change. Data of SDS-PAGE showed obvious degradation of lysozyme branches. 1H-NMR analysis demonstrated that tryptophan（Trp） residue of lysozyme displayed chemical shift after treatment. Conclusion：The conformation structure of lysozyme could be changed by HIFU.

关键词

高强度聚焦超声溶菌酶蛋白结构影响

Keywords

high intensity focused ultrasoundlysozymeprotein structureeffect

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