Dynamic pathological changes in the brain of female APP/PS1 double transgenic mice with different ages

Liu Gang; Liao E; Zhong Guochao; Wang Lingxi; Zhou Zefen; He Guiqiong

您当前的位置：

首页 >

文章列表页 >

Dynamic pathological changes in the brain of female APP/PS1 double transgenic mice with different ages

更新时间：2023-12-18

- Dynamic pathological changes in the brain of female APP/PS1 double transgenic mice with different ages
- Journal of Chongqing Medical University Vol. 40, Issue 1, Pages: 13-17(2015)
- 作者机构：
  
  重庆医科大学神经科学研究中心,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2015，
扫描看全文
Liu Gang, Liao E, Zhong Guochao, et al. Dynamic pathological changes in the brain of female APP/PS1 double transgenic mice with different ages. [J]. Journal of Chongqing Medical University 40(1):13-17(2015)
DOI：

Liu Gang, Liao E, Zhong Guochao, et al. Dynamic pathological changes in the brain of female APP/PS1 double transgenic mice with different ages. [J]. Journal of Chongqing Medical University 40(1):13-17(2015) DOI：

摘要

目的：探讨不同年龄段雌性阿尔茨海默病（Alzheimer’s disease，AD）模型小鼠脑内特征性病理改变的动态变化。方法：选取3月龄、8月龄、12月龄、16月龄雌性APP/PS1双转基因AD模型小鼠各6只，断头取脑后进行石蜡包埋、切片、免疫组化染色，光镜下观察。结果：免疫组化结果显示，3月龄AD模型小鼠脑内神经细胞外偶尔可见β淀粉样蛋白（amyloidβ peptide，Aβ ）沉积形成的老年斑（senile plaque，SP），且SP体积很小；随着年龄的增长，AD模型小鼠脑内SP不仅数量增多，而且体积明显增大[M3=0.600±0.235，M8=8.933±1.730，M12=28.470±1.841，M16=94.130±4.287；P1=0.025，P2=０.000，P3=0.000（M3、M8、M12、M16分别代表3月龄、8月龄、12月龄、16月龄，P1、P2、P3分别代表M3同M8、M8同M12、M12同M16比较所得P值，下同）]；AD模型小鼠脑内神经元标志物NeuN的阳性细胞数量随年龄增长呈进行性下降的趋势（M3=1 919.00±58.21，M8=1 717.00±65.83，M12=1 689.00±45.66，M16=1 529.00±45.36；P1=0.011，P2=0.712，P3=0.043）；而星形胶质细胞（astrocyte，AC）标志物胶质纤维酸性蛋白（glial fibril－lary acidic protein，GFAP）的阳性细胞数量则随着年龄增长不断增多（M3=9.2±6.7，M8=1 053.0±171.3，M12=2 058.0±210.8，M16=3 283.0±240.30；P1=0.000，P2=0.000，P3=0.000）；而促进Aβ生成的关键酶β-分泌酶（β-site APP cleaving enzyme 1，BACE1）的阳性细胞数量随年龄不断增长增多（M3=2 183.00±85.43，M8=3 466.0±142.5，M12=4 555.00±83.23，M16=5 237.00±133.40；P1=０.000，P2=0.000，P3=0.000），蛋白质印迹（Western blot，WB）也得到同样的结果。结论：雌性APP/PS1双转基因小鼠3月龄开始出现典型AD病理改变，随着年龄的增长，病理改变逐渐加重，无逆转迹象。

Abstract

Objective：To discuss the dynamic pathological changes in the brain of female Alzheimer’s disease（AD） mice with differ－ent ages. Methods：Totally 24 APP/PS1 double transgenic mice with AD of 3，8，12，16-month-age were chosen（6 mice for each age group）. Light microscopic observation was performed after Paraffin embedding，sectioning and immunohistochemical staining. Results Immunohistochemical results showed that，amyloidβ peptide（Aβ） and senile plaque（SP） can be occasionally observed. With the in－crease of the age，the volume and number of SP were increased（M3=0.600±0.235，M8=8.933±1.730，M12=28.470±1.841，M16=94.130±4.287；P1=0.025，P2=0.000，P3=0.000）（M3，M8，M12，M16 stands for 3，8，12，16 months respectively；P1，P2，P3 stands for the comparison of P value between M3 and M8，M8 and M12，M12 and M16）. Accordingly，NeuN positive cells in the brain of mice were in a declined trend（M3=1 919.00±58.21，M8=1 717.00±65.83，M12=1 689.00±45.66，M16=1 529.00±45.36，P1=0.011，P2=0.712，P3=0.043），glial fibrillary acidic protein-positive cells were increased（M3=9.2±6.7，M8=1 053.0±171.3，M12=2 058.0±210.8，M16=3 283.0±240.3；P1=0.000，P2=0.000，P3=0.000） and β-site APP cleaving enzyme 1 positive cells were increased（M3=2 183.00±85.43，M8=3 466.00±142.50，M12=4 555.00±83.23，M16=5 237.00±133.40；P1=0.000，P2=0.000，P3=0.000）. Similar results were achieved by Western blot. Conclusion：Female APP/PS1 double transgenic mice display typical AD pathological changes from the age of 3 months to 16 months. The pathological changes grad－ually increase with increasing of age，without reversal.

关键词

阿尔茨海默病老年斑神经元星形胶质细胞β-分泌酶

Keywords

Alzheimer’s diseasesenile plaqueneuronastro－cyteβ-site APP cleaving enzyme 1

references

Views

790

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of Aβ on lysosomes in neurons and microglia of Alzheimer’s disease model mice

Bioinformatics analysis of differentially co-expressed genes in peripheral blood and hippocampal tissue of patients with Alzheimer’s disease

Picroside Ⅱ improves Aβ-induced learning and memory deficits in mice by enhancing autophagy

CX3CL1 improves neuronal function in mice with Alzheimer’s disease by inhibiting microglial pyroptosis

Water extract of Dendrobium nobile Lindl. alleviates learning and memory impairment through synaptic plasticity modulation and glial cell activation in APP/PS1 mice

Related Author

Jiang Lingfang

Zhong Yishi

Wu Yinru

Gao Jian

Song Weiqi

Chen Peiliang

Chen Ziting

Wu Xianbo

Related Institution

Experimental Teaching Management Center, Institute of Neuroscience, Chongqing Medical University

Department of Epidemiology，School of Public Health，Southern Medical University

Chongqing Institute of Medicinal Plant Cultivation， Chongqing College of Traditional Chinese Medicine

Pediatric Research Institute，National Clinical Research Center for Child Health and Disorders， Ministry of Education Key Laboratory of Child Development and Disorders，Chongqing Key Laboratory of Translational Medical Research in Cognitive Development and Learning and Memory Disorders， Children’s Hospital of Chongqing Medical University

Department of General Medicine，Chengdu Second People’s Hospital

⁰