Expression of PI3k/Akt signal pathway and MMP-2，MMP-9 in villi tissues of normal pregnancy and spontaneous abortion

Liu Liyuan; He Junlin; Liu Xueqing; Ding Yubin; Wang Yingxiong; Chen Xuemei

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Expression of PI3k/Akt signal pathway and MMP-2，MMP-9 in villi tissues of normal pregnancy and spontaneous abortion

更新时间：2023-12-18

- Expression of PI3k/Akt signal pathway and MMP-2，MMP-9 in villi tissues of normal pregnancy and spontaneous abortion
- Journal of Chongqing Medical University Vol. 38, Issue 11, Pages: 1511-1515(2014)
- 作者机构：
  
  重庆医科大学公共卫生与管理学院生殖生物学教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2014，
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Liu Liyuan, He Junlin, Liu Xueqing, et al. Expression of PI3k/Akt signal pathway and MMP-2，MMP-9 in villi tissues of normal pregnancy and spontaneous abortion. [J]. Journal of Chongqing Medical University 38(11):1511-1515(2014)
DOI：

Liu Liyuan, He Junlin, Liu Xueqing, et al. Expression of PI3k/Akt signal pathway and MMP-2，MMP-9 in villi tissues of normal pregnancy and spontaneous abortion. [J]. Journal of Chongqing Medical University 38(11):1511-1515(2014) DOI：

摘要

目的：检测磷脂酰肌醇３激酶/蛋白激酶Ｂ（phosphatidylinotidol 3-kinase/protein kinase B，PI3k/Akt）信号通路及基质金属蛋白酶-2（matrix metalloproteinase-2，MMP-2）与基质金属蛋白酶-9（matrix metalloproteinase-9，MMP-9）在早孕期正常妊娠和自然流产绒毛组织中的表达情况，并探讨其表达与自然流产的关系。方法：采用real-time PCR、免疫组织化学方法和Western blot技术检测20例早孕期自然流产绒毛组织与20例正常人工流产绒毛组织中PI3k、Akt、p-Akt、张力蛋白磷酸酶（phosphatase and tensin homolog，PTEN）、MMP-2、MMP-9等表达情况。结果：荧光定量PCR结果显示，PI3k（P=0.000）、Akt（P=0.005）、MMP-9（P=0.000）的 mRNA水平在自然流产组（SPO组）明显低于正常人工流产组（NOR组），PTEN的 mRNA水平在SPO组则高于NOR组（P=0.000）。免疫组化结果显示PI3k（P=0.006）、Akt（P=0.000）、p-Akt（P=0.000）、MMP-9（P=0.000）在SPO组表达明显低于NOR组，PTEN的表达则呈现相反的趋势（P=0.000），MMP-2在SPO组与NOR组没有明显变化（P=0.092）。Western blot 结果与免疫组化结果一致，PI3k（P=0.047）、Akt（P=0.011）、p-Akt（P=0.001）、MMP-2（P=0.893）、MMP-9（P=0.005）。结论：PI3k/Akt信号通路参与了早孕期自然流产的发生，可能通过影响MMP-9的表达而影响其侵袭功能。本研究对于揭示自然流产发生的分子机制具有重要意义，为临床上预防与治疗早期流产的发生提供了新的方向。

Abstract

Objective：To investigate the expression of phosphatidylinotidol 3-kinase/protein kinase B（PI3k/Akt） signal pathway，matrix metalloproteinase-2（MMP-2） and matrix metalloproteinase-9（MMP-9） in villi tissues of normal pregnancy and spontaneous abortion and their relationship. Methods：Real-time PCR，immunohistochemistry and Western blot were used to detect the expression and location of PI3k，Akt，p-Akt，PTEN，MMP-2，MMP-9 between normal artificial abortion（20 cases） and spontaneous abortion（20 cases） in villi tissues. Results：The mRNA expression of PI3k（P=0.000），Akt（P=0.005） and MMP-9（P=0.000） in the villi tissues was higher in normal artificial abortion（NOR group） than in spontaneous abortion（SPO group）. The mRNA expression of PTEN in the villi tissues was higher in SPO group than in NOR group（P=0.000）. The protein expression of PI3k（P=0.006），Akt（P=0.000），p-Akt（P=0.000） and MMP-9（P=0.000） in the villi tissues was higher in NOP group than in SPO group. The protein expression of PTEN was higher in SPO group than in NOR group（P=0.000）. MMP-2 had no significant change between two groups（P=0.092）. Western blot results were consistent with the immunohistochemical results. PI3k（P=0.047），Akt（P=0.011），p-Akt（P=0.001），MMP-2（P=0.893） and MMP-9（P=0.005）. Conclusion：PI3k/Akt signal pathway is involved in the spontaneous abortion during the first trimester，which may affect the expression of MMP-9 and reduce the invasion of tropholast. This study provides new evidence for clinical preven－tion and treatment of early abortion.

关键词

自然流产磷脂酰肌醇３激酶/蛋白激酶Ｂ基质金属蛋白酶-2基质金属蛋白酶-9

Keywords

spontaneous abortionphosphatidylinotidol 3-ki－nase/protein kinase Bmatrix metalloproteinase-2matrix met－alloproteinase-9

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