Expression of HIF-1α and miR-210 in cervical squamous cell carcinoma and their relationship

Lou Meng; Jia Ying

您当前的位置：

首页 >

文章列表页 >

Expression of HIF-1α and miR-210 in cervical squamous cell carcinoma and their relationship

更新时间：2023-12-18

- Expression of HIF-1α and miR-210 in cervical squamous cell carcinoma and their relationship
- Journal of Chongqing Medical University Vol. 40, Issue 4, Pages: 604-606(2015)
- 作者机构：
  
  重庆医科大学附属第一医院妇产科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2015，
扫描看全文
Lou Meng, Jia Ying. Expression of HIF-1α and miR-210 in cervical squamous cell carcinoma and their relationship. [J]. Journal of Chongqing Medical University 40(4):604-606(2015)
DOI：

Lou Meng, Jia Ying. Expression of HIF-1α and miR-210 in cervical squamous cell carcinoma and their relationship. [J]. Journal of Chongqing Medical University 40(4):604-606(2015) DOI：

摘要

目的：探讨宫颈癌组织中缺氧诱导因子-1α（hypoxia induced factor-1α）和MicroRNA 210（miR-210）之间的关系，为宫颈癌的早期发现寻找参考指标。方法：采用免疫组化En Vision法和real-time PCR分别检测52例宫颈鳞癌及20例正常宫颈上皮组织中 HIF-1α和 miR-210的表达情况，用Spearman秩相关检验分析癌组织中HIF-1α和miR-210之间的相关性。结果：宫颈鳞癌组织细胞大部分胞核表达HIF-1α，少部分胞核和胞浆同时表达，HIF-1α在宫颈鳞癌组织中的表达率为73.1%（38/52），正常宫颈组织细胞中无表达。miR-210在宫颈癌组织中的表达水平较正常宫颈组织显著上调，正常宫颈组织miR-210的表达水平为1.16±0.76，宫颈鳞癌组织中其表达水平为5.04±1.28（P=0.000）；在52例宫颈癌组织中，HIF-1α蛋白表达水平与miR-210表达呈正相关（r=0.43，P=0.001）。HIF-1α蛋白在正常宫颈组织中均无阳性表达，与miR-210无相关性。在所有72例样本中进行相关性分析，发现两者的表达呈正相关（r=0.67，P=0.000）。说明随着HIF-1α蛋白表达的增加，miR-210则随着增加。结论：HIF-1α和miR-210在宫颈鳞癌组织中均呈高表达，且两者有一定的相关性。推测HIF-1α和 miR-210与宫颈癌的发生发展相关，有助于进一步揭示宫颈癌的发病机制。

Abstract

Objective：To explore the correlation between hypoxia induced factor-1α（HIF-1α） and MicroRNA210（miR-210） in cer－vical cancer． Methods：The expression of HIF-1α protein was detected by immunohistochemistry. Poly（A）-RT-qPCR was used to de－tect the expression of miR-210 in cervical cancer and normal cervical tissues. The correlation between the expression of miR-210 and HIF-1α was analyzed in cervical cancer. Results：The expression of HIF-1α in cervical cancer was 73.1% （38/52），but HIF-1α was not detected in normal cervical tissues；the expression of miR-210 was significantly increased in cervical cancer tissues than in normal cervical tissues（P=0.000）. The expression of miR-210 in normal cervical tissues and cervical cancer was 1.16±0.76 and 5.04±1.28，respectively. The expression of HIF-1α and miR-210 were positively correlated in 52 cases of cervical cancer（r=0.43，P=0.001）. The expressions of HIF-1α and miR-210 were not correlated in normal cervical tissues. The expressions of HIF-1α and miR-210 were positively correlated in 72 cases（r=0.67，P=0.000）. Conclusion：The expression of HIF-1α and miR-210 are highly expressed in SCC tissues. HIF-1α and miR-210 are positively correlated in cervical cancer. It is predicted that HIF-1α and miR-210 are re－lated with the occurrence and development of cervical cancers，which is conductive to revealing the pathogenesis of cervical cancer.

关键词

宫颈癌缺氧诱导因子-1αmicroRNA210免疫组化real-time PCR

Keywords

cervical cancerhypoxia induced factor-1αmicroRNA210immunohistochemistryreal-time PCR

references

Views

253

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Cervical cancer screening system TruScreen with graph neural network-based classification versus HPV DNA quantification in high-risk HPV-positive patients

Research progress on artificial intelligence in screening and precise diagnosis and treatment of cervical cancer

CXCL10/CCL2 signaling pathway mediates the development of cervical cancer in tumor microenvironment

The diagnostic role of flow cytometry immunophenotyping analysis in 11 cases of aggressive NK-cell leukemia

Expression changes and its significance of SMYD2 in cervical cancer

Related Author

Hu Yanli

Zhou Leming

Huang Zhongxiu

Huang Shixin

Li Xiaosong

Zhu Xuehui

Rong Chao

Li Ang

Related Institution

Department of Gynecology，Qianjiang Hospital，Chongqing University

School of Computer Science and Technology，Chongqing University of Posts and Telecommunications

Department of Clinical Laboratory， Qianjiang Hospital，Chongqing University

Science and Education Department，Yubei District People’s Hospital of Chongqing

Department of Pathology and Pathophysiology，School of Biology & Basic Medical Sciences， Suzhou Medical College of Soochow University

⁰