Effect of IL-28B gene polymorphism and expression on antiviral therapy of patients with chronic hepatitis C

Luo Qi; Wu Chunxia; Li Chengmin; Yuan Qin; Zheng Jie; Liu Feifei; Luo Hongchun; Zeng Aizhong

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Effect of IL-28B gene polymorphism and expression on antiviral therapy of patients with chronic hepatitis C

更新时间：2023-12-18

- Effect of IL-28B gene polymorphism and expression on antiviral therapy of patients with chronic hepatitis C
- Journal of Chongqing Medical University Vol. 40, Issue 3, Pages: 335-339(2015)
- 作者机构：
  
  重庆医科大学附属第一医院感染科、重庆市传染病寄生虫病学重点实验室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2015，
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Luo Qi, Wu Chunxia, Li Chengmin, et al. Effect of IL-28B gene polymorphism and expression on antiviral therapy of patients with chronic hepatitis C. [J]. Journal of Chongqing Medical University 40(3):335-339(2015)
DOI：

Luo Qi, Wu Chunxia, Li Chengmin, et al. Effect of IL-28B gene polymorphism and expression on antiviral therapy of patients with chronic hepatitis C. [J]. Journal of Chongqing Medical University 40(3):335-339(2015) DOI：

摘要

目的：探索白细胞介素28B（interleukin-28B，IL-28B）在慢性丙型肝炎（chronic hepatitis C，CHC）患者抗病毒治疗中的作用。方法：收集到46例CHC患者的抗凝全血，采用多重高温连接酶检测反应检测IL-28B单核甘酸多态性位点rs8099917。同时收集12例健康者血清，46例CHC患者初治时的血清及治疗后12周时其中44例CHC患者的血清，采用ELISA法检测IL-28B水平，并与肝功能进行相关分析。结果：①在46例CHC患者中，rs8099917 TT基因型约占87.0%（40/46），rs8099917 GT基因型约占13.0%（6/46）。血清IL-28B水平在CHC组与健康组，基因1b型与非1b型CHC患者组，高病毒载量与低病毒载量组及rs8099917 TT基因型组与GT基因型组间差别无统计学意义（P均>0.05），与丙氨酸氨基转移酶、门冬氨酸氨基转移酶、谷氨酰转肽酶、血小板之间无相关性。②血清IL-28B在44例CHC治疗后12周达到早期病毒学应答（early virological response，EVR）组比未达到EVR组高，差别有统计学意义（P=0.000）。结论：重庆地区的CHC患者rs8099917基因型以TT型为主。血清IL-28B在rs8099917 TT基因型组与GT基因型组表达虽无差别，但对CHC患者的EVR有重要影响。

Abstract

Objective：To explore the role of interleukin-28B during antiviral treatment in patients with chronic hepatitis C. Methods：Anticoagulant whole blood of 46 patients with chronic hepatitis C were collected.Interleukin-28B single nucleotide polymorphism of rs8099917 genotype was tested by improved multiplex ligation detection reaction.At the same time，102 cases of serum samples were collected，including 46 cases of chronic hepatitis C before treatment and 44 cases of chronic hepatitis C after 12-week therapy，12 cases of healthy controls.The serum levels of interleukin-28B were examined by ELISA and the correlation of interleukin-28B with liver function was analyzed. Results：（1）rs8099917 TT genotype was 87.0%（40/46） and rs8099917 GT genotype was 13.0%（6/46） a－mong the 46 cases of chronic hepatitis C. There was no statistical significance in the serum levels of interleukin-28B between chronic hepatitis C group and healthy group，hepatitis C viral genotype 1b group and non-1b group，high viral load group and low viral load group，rs8099917 TT genotype group and GT genotype group（P>0.05）. There was no correlation between serum interleukin-28B and aspartate aminotransferase，alanine aminotransferase，γ-glutamyl transferase，platelet.（2）There were significant differences in expres－sion of interleukin-28B among 44 patients with chronic hepatitis C after 12-week therapy，which was higher in the early virological response group than in non early virological response group（P=0.000）. Conclusion：The rs8099917 genotypes of patients with chronic hepatitis C are mainly composed by TT genotype in Chongqing area.There is no significant difference in the levels of interleukin-28B between rs8099917 TT genotype group and rs8099917 GT genotype group，but the expression of interleukin-28B have ef－fect on early virological response.

关键词

白细胞介素28B单核苷酸多态性慢性丙型肝炎治疗效应

Keywords

interleukin-28Bsingle nucleotide polymorphismchronic hepatitis Ctherapy efficiency

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