Experimental Study of Effects of Ultrasound Combined with Microbubbles on Apoptosis of A549 Cells Treated by Carboplatin Chemotherapy

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Experimental Study of Effects of Ultrasound Combined with Microbubbles on Apoptosis of A549 Cells Treated by Carboplatin Chemotherapy

更新时间：2023-10-11

- Experimental Study of Effects of Ultrasound Combined with Microbubbles on Apoptosis of A549 Cells Treated by Carboplatin Chemotherapy
- Journal of Clinical Ultrasound in Medicine Vol. 25, Issue 2, (2023)
- 作者机构：
  
  1. 北部战区总医院超声诊断科
  2. 解放军32170部队
  3. 陆军军医大学第二附属医院超声科
  4. 沙坪坝区人民医院超声科
  5. 贵黔国际总医院超声医学中心
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2023，
  
  Published Online：6 March 2023，
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Experimental Study of Effects of Ultrasound Combined with Microbubbles on Apoptosis of A549 Cells Treated by Carboplatin Chemotherapy. [J]. Journal of Clinical Ultrasound in Medicine 25(2).(2023)
DOI：

Experimental Study of Effects of Ultrasound Combined with Microbubbles on Apoptosis of A549 Cells Treated by Carboplatin Chemotherapy. [J]. Journal of Clinical Ultrasound in Medicine 25(2).(2023) DOI：

摘要

目的探讨超声联合微泡是否协同卡铂诱导人肺癌A549细胞凋亡。方法设置对照组、US组、USMB组、CBP组、CBP+US组、CBP+USMB组

其中卡铂采用最适宜剂量50μg/ml

超声辐照参数采用最适宜声强0.6W/cm2

辐照时间60s。处理后培养24小时

每组分别消化、离心、收集3个独立样本

加入FITC标记的Annexin-V室温避光30 min

再加入PI避光反应5 min

应用流式细胞仪检测各组A549细胞凋亡率。应用SPSS 26.0分析各组凋亡率。结果 A549细胞总凋亡率由高到低依次为CBP+USMB组、CBP+US组、CBP组、USMB组、US组。CBP+US组与CBP组比较

早期凋亡率差异无统计学意义(P=0.964)。CBP+USMB组与CBP组、CBP+US组比较

早期凋亡率差异均有统计学意义(均P<0.01)。CBP+US组、CBP+USMB组与CBP组两两比较

晚期、总凋亡率差异均有统计学意义(均P<0.01)。结论 US、USMB联合卡铂化疗可以协同诱导A549细胞凋亡。

Abstract

ABSTRACT Objective To investigate whether ultrasound combined with microbubbles and carboplatin can induce apoptosis of human lung cancer A549 cell line. Methods The control group

US group

USMB group

CBP group

CBP + US group and CBP + USMB group were set

in which carboplatin was used at an optimal dose of 50 μg/ml

ultrasound irradiation was used at an optimal intensity of 0.6 W / cm2

and irradiation time was 60s. After 24 hours of treatment

the cells in each group were collected. FITC-labeled Annexin-V was added for 30 minutes

and then PI was added to protect from light for 5 minutes. Then

flow cytometry was applied. The apoptosis rate of A549 cells was detected. The SPSS 26.0 software was used to analyze the apoptosis rate of each group. Results The total apoptosis rate was ranked as CBP+USMB group>CBP+US group>CBP group>USMB group>US group. There was no significant difference in the early apoptosis rate between the CBP + US and CBP groups (P=0.964). The early apoptosis rates were significantly different between the CBP + USMB group and the CBP group or the CBP + US group (all P < 0.01). Pairwise comparison showed that there were significant differences in the late and total apoptosis rates among the CBP + US

CBP + USMB

and CBP groups (all P<0.01). Conclusion US or USMB combined with carboplatin chemotherapy can synergistically induce the apoptosis of A549 cells.

关键词

超声微泡凋亡空化效应卡铂

Keywords

UltrasoundMicrobubblesApoptosisUltrasonic cavitationCarboplatin

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