In Vitro Experimental Study of Drug-loaded Nanoparticles with Phase-changed Capability for Multimodal Imaging and Sonodynamic Therapy combined with Chemotherapy

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In Vitro Experimental Study of Drug-loaded Nanoparticles with Phase-changed Capability for Multimodal Imaging and Sonodynamic Therapy combined with Chemotherapy

更新时间：2023-10-09

- In Vitro Experimental Study of Drug-loaded Nanoparticles with Phase-changed Capability for Multimodal Imaging and Sonodynamic Therapy combined with Chemotherapy
- Journal of Clinical Ultrasound in Medicine Vol. 23, Issue 10, (2021)
- 作者机构：
  
  1. 重庆医科大学附属第一医院
  2. 重庆医科大学附属第二医院
  3. 重庆医科大学生物医学工程学院
  4. 中国科学院大学重庆医院（重庆市人民医院）
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2021，
  
  Published Online：29 October 2021，
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In Vitro Experimental Study of Drug-loaded Nanoparticles with Phase-changed Capability for Multimodal Imaging and Sonodynamic Therapy combined with Chemotherapy. [J]. Journal of Clinical Ultrasound in Medicine 23(10).(2021)
DOI：

In Vitro Experimental Study of Drug-loaded Nanoparticles with Phase-changed Capability for Multimodal Imaging and Sonodynamic Therapy combined with Chemotherapy. [J]. Journal of Clinical Ultrasound in Medicine 23(10).(2021) DOI：

摘要

目的制备载全氟戊烷/锰卟啉/紫杉醇的多功能纳米探针

评估其体外光声/超声/磁共振三模态成像及声动力治疗(SDT)联合化疗的效果。方法采用双乳化法制备载全氟戊烷(PFP)/锰卟啉(MnTTP)/紫杉醇(PTX)的聚乳酸-羟基乙醇酸(PLGA)纳米粒(PFP-MnTTP-PTX@PLGA

FMP@P)

检测其表面电位、平均粒径、药物包封率

并在透射电镜及扫描电镜下观察其形态。观察纳米粒的体外光声/超声/磁共振显像效果及活性氧产生能力。通过CCK-8法及流式细胞术验证FMP@P体外SDT联合化疗杀伤肿瘤细胞的效果。结果 FMP@P平均粒径为(323.1 ± 68.3)nm

表面电位为(-14.5 ± 8.3)mV

MnTTP及PTX包封率分别为93.55 ± 0.46%、81.23 ± 6.93%。纳米粒可以增强超声/光声/磁共振成像。经超声辐照后

4T1细胞内产生大量活性氧

可显著杀伤肿瘤细胞。结论本研究成功制备了载全氟戊烷/锰卟啉/紫杉醇的多功能纳米粒

可体外增强光声/超声/磁共振显像

并介导SDT联合化学治疗。

Abstract

Objective To prepare poly (lactic-co-glycolic acid) (PLGA) based multifunctional nanoparticles loading with perfluoropentane (PFP)

Mn (5

20-tetrakis (4-chlorophenyl) porphyrin) Cl (MnTTP) and paclitaxel (PTX)

and to investigate their ultrasound (US)/photoacoustic (PA)/magnetic resonance (MR) imaging enhancement capability and chemo-/sonodynamic therapy (SDT) efficacy in vitro. Methods PFP/MnTTP/PTX loaded in PLGA nanoparticles (termed as FMP@P) were prepared by a double emulsification method. The physical properties of FMP@P including average diameter

surface zeta potential

morphological character

drug loading efficacy and in vitro reactive oxygen species (ROS) generation were determined. In addition

the in vitro PA/US/MR imaging performance of FMP@P was observed and recorded. Upon US irradiation

intercellular ROS generation was investigated using confocal microscopy and flowcytometry. Meanwhile

the chemo-/sonodynamic therapy effect on 4T1 cell was evaluated by the standard CCK-8 assay and flow cytometry. Results The average diameter and zeta potential of FMP@P were (323.1 ± 68.3) nm and -(14.5 ± 8.3) mV. The loading efficiency of MnTTP and PTX was 93.55 ± 0.46% and 81.23 ± 6.93%

respectively. FMP@P enhanced US/PA/MR imaging. Upon US irradiation

FMP@P could produce a large amount of reactive oxygen species (ROS)

which induced obvious cytotoxicity. Conclusion A multifunctional FMP@P were successfully fabricated

which enhanced US/PA/ MR imaging

mediated chemo-/SDT.

关键词

光声成像超声成像磁共振成像声动力治疗化疗

Keywords

Photoacoustic imagingUltrasonic imagingMagnetic resonance imagingchemotherapysonodynamic therapy

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