Abstract：Objective To investigate the ultrasonographic characteristics of PTC patients with BRAF V600E mutation and its relationship with lymph node metastasis. Methods Clinical data of 417 patients with PTC confirmed by puncture or surgery histopathology were retrospectively chosen in the period from January 2015 to January 2022. The relationship between the positive rate of BRAF V600E mutation and lymph node metastasis of patients with different subtypes was analyzed and the influence of BRAF V600E mutation on the ultrasonic image characteristics of patients with different subtypes was evaluated by Logistic regression model. Results ①The BRAF V600E mutation positive rate of TCV group was significantly higher than CV group and FV group（P<0.05）. ②There were significant difference in cervical lymph node metastasis rate among different subtypes of BRAF V600E mutation positive population（P<0.05）. The cervical lymph node metastasis rate of BRAF V600E mutation positive patients with different kinds of subtypes were significantly higher than BRAF V600E mutation negative（P<0.05）. ③There were significant difference in the proportion of calcification types and calcification close to the capsule between different kinds of subtypes（P<0.05）. ④Multivariate analysis showed that BRAF V600E mutations were independently associated with calcification of patients with CV and TCV subtypes（P<0.05）. Conclusion PTC patients with BRAF V600E mutation possess the higher incidence of cervical lymph node metastasis and the presence of BRAF V600E mutation can affect the calcification type of ultrasound image in PTC patients with CV/ TCV subtype.