Preparation of HMME-Gd liquid fluorocarbon nanoparticles contrast agent and dual-mode imaging in vitro

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Preparation of HMME-Gd liquid fluorocarbon nanoparticles contrast agent and dual-mode imaging in vitro

更新时间：2023-10-09

- Preparation of HMME-Gd liquid fluorocarbon nanoparticles contrast agent and dual-mode imaging in vitro
- Journal of Clinical Ultrasound in Medicine Vol. 22, Issue 11, (2020)
- 作者机构：
  
  1. 重庆医科大学附属第二医院超声科
  2. 重庆医科大学附属第二医院眼科
  3. 中国科学院大学重庆医院（重庆市人民医院）全科医学科
  4. 重庆医科大学附属第二医院神经外科
  5. 重庆市超声影学研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2020，
  
  Published Online：1 December 2020，
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Preparation of HMME-Gd liquid fluorocarbon nanoparticles contrast agent and dual-mode imaging in vitro. [J]. Journal of Clinical Ultrasound in Medicine 22(11).(2020)
DOI：

Preparation of HMME-Gd liquid fluorocarbon nanoparticles contrast agent and dual-mode imaging in vitro. [J]. Journal of Clinical Ultrasound in Medicine 22(11).(2020) DOI：

摘要

目的制备携带血卟啉单甲醚-钆(HMME-Gd)的液态氟碳纳米粒(HMME-Gd-PFPNPs)

观察体外US/MRI的成像效果。方法以磷脂、胆固醇、HMME-Gd和全氟戊烷(PFP)为原料

用薄膜水化法和乳化法制备脂质体纳米粒HMME-Gd-PFPNPs。倒置光学显微镜、透射电子显微镜及激光共聚焦显微镜检测其基本表征；粒径分析仪分析粒径及表面电位；紫外分光光度计分析HMME-Gd的包封率；观察HMME-Gd-PFPNPs体外US/MRI成像的效果。结果成功制备HMME-Gd-PFPNPs

光镜下为形态规则、大小均一的球形

电镜下为黑色的球形结构。HMME-Gd-PFPNPs平均粒径为(250.27 ± 11.9)nm

电位为(-26.17 ± 0.45)mV

HMME-Gd的包封率为(84.7 ± 0.35)%。在低强度聚焦超声辐照下

体外US成像信号显著增强

与LIFU激发强度成正相关。随HMME-Gd-PFPNPs浓度增加

体外MRI成像效果显著增强。结论成功制备了携带HMME-Gd的液态氟碳纳米粒

可用于体外US/MRI双模态成像。

Abstract

Objective To prepare liquid fluorocarbon nanoparticles carrying HMME-Gd (HMME-Gd-PFPNPs) and observe the effect of ultrasound/MRI imaging capability in vitro. Methods Lipids cholesterol

hematoporphyrin monomethyl ether gadolinium (HMME-Gd) and perfluoropentane (PFP) were used as raw materials to prepare nanoparticles by membrane hydration and emulsification. The general physical properties were measured by optical microscopy

transmission electron microscopy and laser confocal microscope. The size and surface potential were analyzed by particle size analyzer. The encapsulation efficiency of HMME-Gd in HMME-Gd-PFPNPs was determined by UV-vis. The ultrasound (US)/magnetic resonance imaging (MRI) capability of HMME-Gd-PFPNPs was observed in vitro. Results HMME-Gd-PFPNPs were successfully prepared

which were regular in shape and uniform in size. They showed a black spherical structure under transmission electron microscopy. The size of HMME-Gd-PFPNPs was (250.27 ± 11.9) nm

the surface potential was (-26.17 ± 0.45) mV

and the encapsulation efficiency of HMME-Gd was (84.7 ± 0.35) %. As the concentration of HMME-Gd-PFPNPs increased

the MRI signal was significantly enhanced in vitro. Under low intensity focused ultrasound (LIFU) irradiation

the US imaging signal was significantly enhanced in vitro

which was positively correlated with the intensity of LIFU. Conclusion The liquid fluorocarbon nanoparticle contrast agent carrying HMME-Gd was successfully prepared and can be used for US/MRI dualmodal imaging in vitro.

关键词

纳米粒造影剂超声成像磁共振成像

Keywords

NanoparticlesContrast agentUltrasound imagingMagnetic resonance imaging

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