Effect of amelogenin on the migration, adhesion and proliferation of periodontal ligament stem cells

ZHAO Xi; XIONG Yu

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Effect of amelogenin on the migration, adhesion and proliferation of periodontal ligament stem cells

更新时间：2023-05-22

- Effect of amelogenin on the migration, adhesion and proliferation of periodontal ligament stem cells
- null Issue 5, (2016)
- 作者机构：
  
  1. 第三军医大学西南医院口腔科
  2. ,重庆,400038
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2016，
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ZHAO Xi, XIONG Yu. Effect of amelogenin on the migration, adhesion and proliferation of periodontal ligament stem cells. [J]. null (5).(2016)
DOI：

ZHAO Xi, XIONG Yu. Effect of amelogenin on the migration, adhesion and proliferation of periodontal ligament stem cells. [J]. null (5).(2016) DOI：

摘要

[摘要]目的探究釉原蛋白(AML)对牙周膜干细胞（PDLSCs）迁移、粘附和增殖的影响。方法流式细胞仪分选Stro-1+ PDLSCs，采用Transwell法检测AML对PDLSCs迁移的影响，粘附实验检测AML对PDLSCs粘附的影响，MTT检测AML对PDLSCs增殖能力的影响。结果Transwell结果显示，AML能够作用于PDLSCs，促进其迁移，并且此影响呈正相关；粘附实验结果显示，PDLSCs的粘附同样受AML的影响，该作用随培养时间延长而增加；MTT结果显示，AML能够促进PDLSCs增殖，且随着剂量增加该促进作用增强。结论体外AML能够作用于PDLSCs，促进其迁移、粘附和增殖。

Abstract

Abstract: ObjectiveTo study the effect of amelogenin (AML) on the migration

adhesion and proliferation of periodontal ligament stem cells (PDLSCs).MethodsSTRO-1+PDLSCs were selected by Flow cytometer.The transwell system was used to detect the effect of AML on migration of PDLSCs. Cells attachment assay was used to detect the effect of AML on adhesion of PDLSCs.MTT was used to detect the effect of AML on the proliferation of PDLSCs.ResultsThe results of transwell showed that AML could induce the migration of PDLSCs with positive correlation.Cells attachment assay showed that the adhesion of PDLSCs was effected by AML as well

and the effect was increased with the extension of incubation time.Results of MTT showed that AML could promote the proliferation of PDLSCs in a dose dependent manner.ConclusionAML could increase the proliferation

adhesion and migration potential of PDLSCs.K

关键词

肺冲击伤幼兔动物模型生物激波管

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