Characteristics and significance of interferon-induced protein with tetratricopetide repeats 1 expressed in radiation injury and infection stress

GUO Xiao-shu; HAO Yu-hui; SU Yong-ping; WANG Jun-ping; WANG Song; XU Yang

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Characteristics and significance of interferon-induced protein with tetratricopetide repeats 1 expressed in radiation injury and infection stress

更新时间：2023-05-22

- Characteristics and significance of interferon-induced protein with tetratricopetide repeats 1 expressed in radiation injury and infection stress
- null Issue 5, (2016)
- 作者机构：
  
  1. 第三军医大学全军复合伤研究所，创伤、烧伤与复合伤国家重点实验室,重庆,400038
  2. 长治医学院生理教研室,山西,长治,046000
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2016，
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GUO Xiao-shu, HAO Yu-hui, SU Yong-ping, et al. Characteristics and significance of interferon-induced protein with tetratricopetide repeats 1 expressed in radiation injury and infection stress. [J]. null (5).(2016)
DOI：

GUO Xiao-shu, HAO Yu-hui, SU Yong-ping, et al. Characteristics and significance of interferon-induced protein with tetratricopetide repeats 1 expressed in radiation injury and infection stress. [J]. null (5).(2016) DOI：

摘要

摘要］目的探讨IFIT1（Interferon-induced protein with tetratricopetide repeats 1）在放射损伤与感染应激中表达的特点和意义。方法采用5 μg/mL的脂多糖（LPS）刺激Raw264.7、3T3和10T1/2细胞株5 h后，收集细胞，提取RNA，行RT-PCR观察IFIT1的表达情况，同时设定空白对照组。另外，将20只C57/BL6小鼠随机分为4组，采用12 Gray60Co一次性全身照射，分别于0 h、1 h、4 h、和12 h脱颈处死取肝组织，用western blot 测定肝组织IFIT1表达变化。结果Raw264.7、3T3和10T1/2细胞株经LPS刺激5 h后，均能诱导IFIT1表达，空白对照组表达为阴性。C57/BL6小鼠放射性损伤后，1 h IFIT1/Actin开始增高，12 h达最高，呈上升趋势，差异有极显著性统计学意义（P<0.01）。结论LPS可刺激多种细胞株表达IFIT1，提示IFIT1可能参与创伤后毒血症的发生和发展；放射损伤早期能引起小鼠肝组织IFIT1 的显著增高。

Abstract

Abstract:ObjectiveTo explore characteristics and significance of interferon-induced protein with tetratricopetide repeats 1 expressed in radiation injury and infection stress.MethodsRNA was extracted from Raw264.7 cell

3T3 cell and 10T1/2 cell after 5 hours stimulated with 5 μg/mL LPS. At the same time

to set up normal control group (untreated by LPS)

and RNA of IFIT1 was detected by RT-PCR.Totally 20 C57/BL6 mice were randomly divided into 4 groups

namely 0 h group

1 h group

4 h group and 12 h group. The mice were given 12 Gray60Co full-body exposure once

then liver IFIT1 was detected by western blot.ResultsStimulated with LPS for 5 hours

IFIT1 was induced expression in Raw264.7 cell

3T3 cell and 10T1/2 cell. The expression of normal control group was negative.The level of IFIT1/Actin increased significantly 1 hour after radiation injury

and it reached the peak 12 hours after radiation injury (P＜0.01).ConclusionLPS can stimulated a variety of cell lines expressed IFIT1

prompting that IFIT1 may participate in the occurrence and development of post-traumatic toxemia.IFIT1 of liver tissue increased significantly during the early stage in radiation mice.

关键词

放射性损伤脂多糖IFIT1糖皮质激素受体毒血症

Keywords

radiation injurylipopolysaccharideIFIT1glucocorticoid receptorstoxemia

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