Effects and mechanisms of wogonin on proliferation and invasion of skin squamous-cell carcinoma cell line A431

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Effects and mechanisms of wogonin on proliferation and invasion of skin squamous-cell carcinoma cell line A431

更新时间：2023-05-22

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- Effects and mechanisms of wogonin on proliferation and invasion of skin squamous-cell carcinoma cell line A431
- null Issue 4, Pages: 256(2019)
- 作者机构：
  
  重庆涪陵中心医院烧伤整形科,重庆,408000
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2019，
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LI Bo, CHEN Zhi-yong, LIU Tai-yang, et al. Effects and mechanisms of wogonin on proliferation and invasion of skin squamous-cell carcinoma cell line A431. [J]. null (4):256(2019)
DOI：

LI Bo, CHEN Zhi-yong, LIU Tai-yang, et al. Effects and mechanisms of wogonin on proliferation and invasion of skin squamous-cell carcinoma cell line A431. [J]. null (4):256(2019) DOI：

摘要

目的探讨汉黄芩素对皮肤鳞癌细胞的增殖和侵袭能力的影响及其潜在作用机制。方法检测正常皮肤组织和皮肤鳞癌组织中Notch1 mRNA和蛋白表达水平。使用0、5、10、和20 μmol/L浓度的汉黄芩素处理皮肤鳞癌A431细胞系24、48、72 h，分别用MTT 法检测各组细胞增殖能力;使用0、5、10、和20 μmol/L浓度的汉黄芩素处理细胞48 h，随后采用Transwell侵袭试验检测各组细胞侵袭能力，Real time PCR(RT-PCR)和Western blot 法检测Notch1基因的mRNA及蛋白表达。使用Notch1激活剂多西环素处理后，进一步采用MTT 法检测各组细胞增殖能力、Transwell法检测各组细胞侵袭能力。结果与正常皮肤组织相比，皮肤鳞癌组织中Notch1基因的mRNA及蛋白表达水平均显著升高，差异具有统计学意义（P＜0.05）。汉黄芩素能够抑制皮肤鳞癌细胞的增殖和侵袭，且呈剂量依赖性，差异具有统计学意义（P＜0.05）。汉黄芩素能够抑制Notch1基因的表达，而应用Notch1激活剂多西环素处理后，明显减弱了汉黄芩素对细胞增殖与侵袭能力的抑制作用，差异具有统计学意义（P＜0.05）。结论汉黄芩素能够抑制皮肤鳞癌细胞的增殖及侵袭进程，可能主要通过抑制Notch1基因来发挥作用。

Abstract

Objective To investigate the role of wogonin on proliferation and invasion of skin squamouscell carcinoma and to explore the potential mechanism. Methods The mRNA and protein expression levels of Notch1 were detected in normal skin tissues and skin squamouscell carcinoma tissues. Skin squamouscell carcinoma cell line A431 were treated with 0

5

10 and 20 μmol/L wogonin for 24

48

72 hours. Cell proliferation capacities were detected by MTT assay. Cell invasion abilities were monitored by Transwell invasion assay. The mRNA and protein expression of Notch1 gene were detected by Real time PCR (RT-PCR) and Western blot. After treatment with Notch1 activator doxycycline

cell proliferation ability and invasion ability of each group were further detected by MTT assay and Transwell assay. Results Compared with normal skin tissue

mRNA and protein expression levels of Notch1 gene in skin squamous cell carcinoma were significantly increased

with statistically significant differences (P＜0.05). Wogonin inhibited the proliferation and invasion ability of skin squamouscell carcinoma cells in a dose dependent manner

with statistically significant differences (P＜0.05). Wogonin inhibited the expression of Notch1 gene

and the treatment with Notch1 activator doxycycline significantly reduced the inhibitory effect of Wogonin on cell proliferation and invasion

with statistically significant differences (P＜0.05). Conclusion Wogonin can inhibite the proliferation and invasion of skin squamouscell carcinoma primarily by inhibiting the Notch1 gene.

关键词

皮肤鳞癌汉黄芩素Notch1增殖侵袭

Keywords

skin squamouscell carcinomaWogoninNotch1proliferationinvasion

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