Effect of exosomes on pathogenesis of ⅢA chronic prostatitis and its significance

QIAO yang; WANG Yongquan; SHEN Wenhao; ZHANG Heng; ZHOU Zhansong

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Effect of exosomes on pathogenesis of ⅢA chronic prostatitis and its significance

更新时间：2023-05-22

- Effect of exosomes on pathogenesis of ⅢA chronic prostatitis and its significance
- null Issue 7, Pages: 516(2019)
- 作者机构：
  
  陆军军医大学第一附属医院泌尿外科,重庆,400038
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2019，
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QIAO yang, WANG Yongquan, SHEN Wenhao, et al. Effect of exosomes on pathogenesis of ⅢA chronic prostatitis and its significance. [J]. null (7):516(2019)
DOI：

QIAO yang, WANG Yongquan, SHEN Wenhao, et al. Effect of exosomes on pathogenesis of ⅢA chronic prostatitis and its significance. [J]. null (7):516(2019) DOI：

摘要

目的探讨前列腺液来源的外泌体在ⅢA型慢性前列腺炎发病机制中的作用。方法选取我院2018年4月至2018年8月收治的30例ⅢA型慢性前列腺炎患者作为ⅢA组，14例健康志愿者作为对照组。利用超速离心法从前列腺按摩后尿液中提取外泌体。通过透射电镜和Western blot鉴定提取的外泌体，使用激光粒度仪测量外泌体粒径，应用外泌体测量试剂盒（ExoELISAULTRA CD631）及BCA法测定外泌体浓度及外泌体蛋白浓度。结果电镜及Western blot显示，从前列腺按摩后尿液中成功提取出外泌体。对照组外泌体平均粒径为(157.5±2.27) nm，ⅢA组外泌体平均粒径为(155.4±1.73) nm，2组比较差异无统计学意义（t=0.7，P>0.05）。对照组外泌体浓度为（6.13±1.19）×1013/L，ⅢA组为（9.77±1.05）×1013/L，2组比较差异有统计学意义（t=2.09，P<0.05）。对照组外泌体蛋白浓度为（1.24±0.16） g/L，ⅢA组为（1.84 ±0.17） g/L，2组比较差异有统计学意义（t=2.18，P<0.05）。结论 ⅢA组前列腺按摩后尿液中外泌体数量多于对照组，外泌体可能在ⅢA型慢性前列腺炎发病机制中具有重要作用。

Abstract

Objective To investigate the effect of exosomes in the pathogenesis of ⅢA chronic prostatitis(CP). Methods A total of 30 patients with ⅢA CP in our hospital from April to August 2018 were selected as ⅢA group

and 14 healthy people were selected as control group.Differential ultracentrifugation was performed to isolate exosomes from the expressed prostatic secretion in urine(EPSurine).Exosomes were characterized by transmission electron microscopy and Western blot.The size of exosomes were analyzed by particulate size description analyser.Exosomes were quantified using Exosome Quantification Assay Kit（ExoELISAULTRA CD631）.Proteins were quantified by BCA assay. Results Exosomes were successfully isolated from EPSurine and identified by transmission electron microscopy and Western blot. The average size of exosomes in control group was (157.5±2.27)nm

that in ⅢA group was (155.4±1.73)nm

the difference was not significant（t=0.7，P>0.05）.The exosomes concentration in control group was (6.13±1.19)×1013/L

that in ⅢA group was (9.77±1.05)×1013/L

the difference was significant（t=2.09，P<0.05）.The protein concentration in control group was (1.24±0.16)g/L

that in ⅢA group was (1.84±0.17)g/L

the difference was significant（t=2.18，P<0.05）.Conclusion The level of exosomes in ⅢA group was significantly higher than that in control group.Exosomes may have an important role in the etiology of ⅢA CP.

关键词

ⅢA型慢性前列腺炎外泌体前列腺按摩后尿液CD9CD63

Keywords

ⅢA chronic prostatitisexosomeexpressed prostatic secretion in urineCD9CD63

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