Objective To evaluate the effects of high glucose on the apoptosis and oxidative stress of osteoblasts MC3T3E1

and to provide a certain experimental basis for clinical treatment of diabetic osteoporosis. Methods Osteoblasts MC3T3E1 were cultured in cell culture bottles with different concentrations of high glucose solutions (0

20

40

80 mmol/L) for 24 hours.CCK8 was used to determine the cell survival rate and apoptosis rate

mitochondrial membrane potential kit was used to measure the degree of depolarization

caspase3 kit was used to detect its activity

and DCFHDA fluorescence probe was used to detect the generation of ROS.The mitochondrial membrane potential and caspase3 content of MC3T3E1 cells were measured by the above method under the action of antioxidant NAC at 80 mmol/L high glucose exposure. Results Compared with the control group

osteoblasts MC3T3E1 exposed to high glucose showed decreased cell survival rate and mitochondrial membrane potential

enhanced apoptosis rate

ROS level and caspase3 activity

and dosedependent tolerance.The antioxidant NAC inhibited the mitochondrial membrane potential decrease and caspase3 increase of osteoblasts MC3T3E1 induced by high glucose. Conclusion In high glucose environment

NAC can inhibit oxidative stress induced by high glucose

reduce apoptosis rate and protect osteoblasts MC3T3E1.