OBJECTIVE： To study sterility test after using Non-PVC bivalve soft-bag injection in PIVAS. METHODS： The test was divided into 3 groups according to the type of transfusion solution packaging and dispensing environment. Group 1 received Glucose solution using bivalve soft-bag， dispensed in PIVAS； group 2 received Glucose solution using bivalve soft-bag， dispensed in wards area； group 3 received Glucose solution using plastic bottle， dispensed in wards area. After puncturing 1， 3， 6， 9 times （n＝80）， finished products placed in ward for 0， 2， 4， 6 h （n＝20）， and then sterility test was conducted with membrane filtration method stated in second part of Chinese Pharmacopoeia （2010 edition）. Infusion contamination of 3 groups was analyzed at 9th puncture. RESULTS： The growth of bacteria was not found in group 1； the positive detection rate of group 2 and 3 were 2.5％ and 3.8％ （n＝320）. The total positive detection rates after puncturing 1， 3， 6， 9 times were 0， 0.4％， 0.4％， 7.5％ （n＝240）； the positive detection rates of group 1 were all 0， those of group 2 were 0，1.25％，0，8.75％ and those of group 3 were 0，0，1.25％，13.75％ （n＝80）. After 9 times of puncture， the positive detection rates of group 1 after placing 0， 2， 4， 6 h were all 0， those of group 2 were 25％，5％，0，5％； those of group 3 were 5％，15％，5％，30％ （n＝20）. CONCLUSIONS： The use of the Non-PVC bivalve soft-bag injection in PIVAS can effectively prevent microbial contamination.