OBJECTIVE： To screen Gongshisong’s active sites for anti-sports fatigue. METHODS： Gongshisong extract was prepared with 80％ ethanol extraction technology， and extracted with petroleum ether， chloroform， ethyl acetate and n-butyl alcohol after dispersed with water to obtain the extract. 70 mice were randomly divided into blank control group （1％ sodium carboxymethylcellulose， CMC-Na）， positive control group [Rhodiola wallichiana capsules， 590 mg/（kg·d）]， petroleum ether， chloroform， ethyl acetate and n-butyl alcohol extracts and aqueous layer of Gongshisong groups （TS， TL， TY， TZ， TW groups）. Gongshisong extracts groups was given relevant medicine 2.5 g（crude drug）/（kg·d）， ig， for consecutive 7 days. Exhaustion time of burden swimming test was detected. 70 mice were grouped according to above method， and the contents of liver glycogen， muscle glycogen and the coefficient of liver were tested in mice. 80 mice were grouped according to above method， and model group was established additionally （1％ CMC-Na）. The contents of lactic acid （LA）， creatine kinase （CK） and urea nitrogen （BUN） in serum of mice were determined after 90 minutes of unburden swimming. RESULTS： Compared with blank control group， exhaustion time of burden swimming mice in TS， TY and TZ groups prolonged； the content of liver glycogen increased in TY， TZ and TW groups； the content of muscle glycogen increased in TS and TW groups； the contents of BUN， LA and CK in mice increased in model group （P＜0.05 or P＜0.01）. Compared with model group， the serum content of BUN in mice decreased in TS and TY groups； that of LA in mice decreased in TZ and TW groups； that of CK in mice decreased in TS group （P＜0.05 or P＜0.01）. CONCLUSIONS： The petroleum ether and n-butanol extract site and water layer of Gongshisong are good anti-fatigue active sites.