OBJECTIVE： To provide reference for rational use of antibacterial drugs in the clinic. METHODS： In retrospective study， outpatient and emergency antibacterial drugs prescriptions were randomly selected from a hospital during 2013-2014， and then analyzed statistically. DDDs of antibacterial drugs were analyzed by using DDD method. RESULTS： From 2013-2014， the application rate of antibacterial drugs in the hospital declined substantially from 13.8％ to 11.9％， and the rate of irrational use dropped from 2.7％ to 1.2％. The application rate of antibacterial drugs was higher in pediatric department， emergency department and urology department； drug cost per capita was higher in infectious department， nephrology department and emergency department. The application rate of antibacterial drugs in pediatric department decreased greatly in 2014， compared with 2013， maintaining about 40％. The application rate of antibacterial drugs in emergency department during Jan.-Sept. in 2014 was lower than corresponding period in 2013， and that of the hospital during Jan.-Sept. in 2014 was lower than corresponding period in 2013， but during Oct.-Dec. in 2014 was slightly higher than corresponding period in 2013. Single type of antibacterial drugs were mainly used in outpatient department， accounting for 91.50％ and 90.77％ of antibacterial drug prescriptions in 2013 and 2014； two-drug accounted for 8.44％ and 9.11％. The top 3 antibacterial drugs in the list of DDDs during 2013-2014 were roxithromycin， cefuroxime and cefminox. CONCLUSIONS： The use of antibacterial drugs is up to the requirements of Nation Special Rectification Actirity for Clinical Application of Antibacterial Drugs in 2013. The prescription comment improve the quality of antibacterial drug prescriptions and rational use index greatly in outpatient department， but the application rate of antibacterial drugs is too high in some department. There still is irrational use of antibacterial drugs.