OBJECTIVE：To establish a method for the 5 main components （original syringin， chlorogenic acid， eleutheroside E， isofraxidin and quercetin-3-rhamnoside） in the fruits and roots of wild Acanthopanax senticosus. METHODS：UPLC was performed on the column of Waters ACQUITY UPLC HSS T3 with mobile phase of acetonitrile-0.3％ phosphoric acid （gradient elution） at a flow rate of 0.2 ml/min. Detection wavelength was 300 nm， column temperature was 30 ℃，and injection volume was 10 μl. RESULTS： The linear range was 24.56-184.2 μg/ml for syringin （r＝0.999 3）， 18.454-138.405 μg/ml for chlorogenic acid （r＝0.999 3），8.416-63.12 μg/ml for eleutheroside E （r＝0.999 7）， 3.286-24.645 μg/ml for isofraxidin （r＝0.999 3） and 2.522-18.915 μg/ml for quercetin-3-rhamnoside（r＝0.999 8）； RSDs of precision， stability and reproducibility tests were lower than 1％； recoveries were 99.14％-100.50％（RSD＝0.48％，n＝6）for syringing in the fruits of A. senticosus、99.03％-100.45％（RSD＝0.50％，n＝6） for chlorogenic acid in the fruits of A. senticosus、99.22％-100.44％（RSD＝0.44％，n＝6）for  eleutheroside E in the fruits of A. senticosus、99.80％-100.80％（RDS＝0.44％，n＝6）for isofraxidin in the fruits of A. senticosus、 99.76％-101.10％（RSD＝0.51％，n＝6）for quercetin-3-rhamnoside in the fruits of A. senticosus；99.21％-101.20％（RSD＝0.73％，n＝6） for syringing in the root of A. senticosus、99.81％-101.20％（RSD＝0.52％，n＝6） for chlorogenic acid in the root of A. senticosus、 100.00％-101.50％（RSD＝0.62％，n＝6）for  eleutheroside E in the root of A. senticosus、99.22％-100.40％（RSD＝0.47％，n＝6）for  isofraxidin in the root of A. senticosus. CONCLUSIONS：The method is simple and stable with good reproducibility， and can be used for the simultaneous determination of original syringin， chlorogenic acid， eleutheroside E， isofraxidin and quercetin-3-rhamnoside in the fruits and root of wild A. senticosus.