OBJECTIVE： To provide reference for improving teaching effects of Medicinal Chemistry Experiment， and cultivating practical talents that in line with needs of the pharmaceutical industry. METHODS： Based on the training target of medicinal preparation major in our college， the basic idea of establishing Medicinal Chemistry Experiment system was discussed， and teaching target， content， process and experimental evaluation results of Medicinal Chemistry Experiment were analyzed in detail. RESULTS： The experiment teaching system was established， with teaching target of improving knowledge， ability and quality， teaching contents of basic experiment， comprehensive experimental design experiment and open experiment， center of developing practical teaching from before class， in class and after class， and means of diversified performance evaluation. CONCLUSIONS： The established experiment teaching system is suitable for the teaching of Medicinal Chemistry Experiment in medicinal preparation major， it has created a new situation that professional， innovation and quality education organic integrate in teaching activity， mobilized the students’ learning enthusiasm and initiative， exercised the students' professional knowledge and practical ability， cultivated students' creative ability and comprehensive quality and enhanced the effect of experiment teaching.