OBJECTIVE: To study the hemostatic effect and mechanism of the polysaccharides from Bletilla striata in blood heat and hemorrhage (BBH) model rats. METHODS: Rats were randomly divided into normal control group, model group, Yunnan baiyao group (0.50 g/kg), and BSP low-dose, medium-dose and high-dose groups (0.34,0.68, 1.35 g/kg), with 10 rats in each group. Except for normal control group, those groups were given water decoction of Rhizoma Zingiberis and 5% ethanol to induce BBH model. After modeling, those groups were given relevant medicine intragastrically, once a day, for 14 days, except normal control group and model group were given normal saline intragastrically. 1 h after last administration, body weight, food intake, water intake, the rate of platelet aggregation, the contents of t-PA and PAI-1, pathological change of lung and stomach were all detected in each group. RESULTS: Compared with normal control group, body weight, food intake, maximal rate of platelet aggregation and the content of PAI-1 were decreased in model group; while water intake, the content of t-PA and ratio of t-PA/PAI-1 were increased (P<0.05 or P<0.01). Moreover, hemorrhage and inflammation of lung and stomach were found. Compared with model group, the body weight and food intake of Yunnan baiyao group, BSP low-dose, medium-dose and high-dose groups were increased, while water intake were decreased (P<0.05 or P<0.01); the maximal rate of platelet aggregation and the content of PAI-1 were increased in Yunnan baiyao group, BSP medium-dose and high-dose groups, while ratio of t-PA/PAI-1 were decreased (P<0.05 or P<0.01), the pathological changes of lung and stomach relieved to varying degrees. CONCLUSIONS: BSP exerts hemostatic effect by up-regulating the rate of platelet aggregation, rising fibrinolysis systems, improving the lesion of lung and stomach.
关键词
白及多糖血热出血大鼠血小板聚集纤溶系统组织病理学检查
Keywords
Polysaccharides from Bletilla striataBlood heat and hemorrhageRatsPlatelet aggregationFibrinolytic systemHistopathological examination