OBJECTIVE： To establish a method for the simultaneous determination of acteoside， jionoside B1， dehydrotumulosic acid， polyporenic acid C， dehydropachymic acid and pachymic acid in Duzhong butiansu tablet. METHODS： HPLC was performed on the column of Kromasil C18 with mobile phase of acetonitrile-0.2％ phosphate acid （gradient elution） at a flow rate of 0.8 mL/min， the detection wavelength was 330 nm for acteoside and jionoside B1， 210 nm for dehydrotumulosic acid， polyporenic acid C， dehydropachymic acid and pachymic acid， the column temperature was 35 ℃， and the injection volume was 10 μL. RESULTS： The linear range was 3.71-74.20 μg/mL for acteoside（r＝0.999 9），4.35-87.00 μg/mL for jionoside B1（r＝0.999 5）， 3.86-77.20 μg/mL for dehydrotumulosic acid （r＝0.999 9），5.05-101.00 μg/mL for polyporenic acid C （r＝0.999 1） and 4.20-84.00 μg/mL for dehydropachymic acid （r＝0.999 7） and 4.73-87.40 μg/mL for pachymic acid （r＝0.999 6）； the limits of qu- antification were 0.322， 0.187， 0.105， 0.381， 0.214， 0.452 μg/mL， and the limits of detection were 0.108， 0.059， 0.032， 0.131， 0.072， 0.149 μg/mL； RSDs of precision， stability and reproducibility tests were lower than 2.0％； recoveries were 96.20％-99.53％（RSD＝1.23％，n＝6）， 96.99％-100.67％（RSD＝1.47％，n＝6），96.64％-100.08％（RSD＝1.28％，n＝6），97.47％-100.59％（RSD＝1.18％，n＝6），97.97％-100.83％（RSD＝1.25％，n＝6） and 96.81％-99.61％（RSD＝1.09％，n＝6）. CONCLUSIONS： The method is simple， rapid， accurate， and can be used for the simultaneous determination of acteoside， jionoside B1， dehydrotumulosic acid， polyporenic acid C， dehydropachymic acid and pachymic acid in Duzhong butiansu tablet.