OBJECTIVE： Study the protective effect of the polypentenol from Ginkgo biloba leaf （GP）on Aβ25-35-induced damage in dPC12 cells ，and to provide reference for the treatment of Alzheimer’s disease （AD）. METHODS：After PC12 cells were differentiated into dPC12 cells with neural activity by incubating with never growth factor （NGF）， the dPC12 cells were divided into normal control group （DMSO medium），Aβ25-35 treatment group （DMSO medium） and GP test group （DMSO medium respectively containing 25，50，100，200，400 μg/mL GP）. After 24 h cultivating， cells in Aβ25-35 treatment group and GP test group were added 25 μmol/L Aβ25-35 to induce cell damage （AD cell model）， MTT method was used to determine the survival rate after 24 h. Other cells were divided into normal control group （DMSO medium）， Aβ25-35 treatment group（DMSO medium）and GP test group（DMSO medium respectively containing 25，50，100，200 μg/mL GP）， the lactate dehydrogenase （LDH）， reactive oxygen species （ROS）， malondialdehyde （MDA） levels in cell culture medium were detected after the same treatment. RESULTS：Compared with Aβ25-35 treatment group， the survival rates of dPC12 cells in 50， 100， 200， 400 μg/mL GP test groups were obviously increased （P＜0.05 or P＜0.01）； LDH， ROS， MDA levels in 100， 200 μg/mL GP test groups and ROS level in 50 μg/mL GP test group were significantly reduced （P＜0.05 or P＜0.01），showing a certain concentration-dependence. CONCLUSIONS： GP has certain protective effect on Aβ25-35-induced damage in dPC12 cells， and it’s a potential drug for AD.