OBJECTIVE： To provide reference for rational use of Iron sucrose injection. METHODS： Refrring to related literatures and gaideline， through pharmacy and clinical expert discussion and revision， DUE standard for Iron sucrose injection was established from medication indication， medication duration and medication results， the intervention measures were formulated and implemented. 56 patients were randomly collected from top 5 departments in the list of total amount of Iron sucrose injection in Feb. 2016 as pre-intervention group； discharged patients were randomly collected from relevant department in Apr. and Jun. 2016 as first stage intervention group （40 cases） and second stage intervention group （53 cases）， respectively. The proportion of Iron sucrose injection meeting the criteria as well as rationality were compared before and after intervention， respectively. RESULTS： After two stages of intervention， pre medication monitoring serum ferritin-related iron deficiency indexes， solvent dosage selection， DDDs， the proportion of anaemia improvement or anaemia-related indexes improvement meeting the criteria in the first stage intervention group and second stage intervention group were all significantly higher than pre-intervention group， with statistical significance （P＜0.05）. The proportion of patient interaction meeting the criteria in the second stage intervention group was significantly higher than first stage intervention group， with statistical significance （P＜0.05）. The comprehensive rational rate of second stage intervention group was 77.34％， which was significantly higher than 41.07％ of pre-intervention group and 47.50％ of first stage intervention group， with statistical significance （P＜0.05）. CONCLUSIONS： DUE standard for Iron sucrose injection have great operability and practicability. Clinical pharmacists’intervention effectively dispose the problems of clinical drug use， promote the rationality of clinical drug use and reduce the incidence of ADR.