OBJECTIVE： To provide reference for rational use of antibiotics in the clinic of our hospital. METHODS： Drug resistance of Gram-negative bacillus in the inpatients of our hospital were analyzed retrospectively during May 2013-Dec. 2015 as well as the situation of producing metallo-β-lactamase （MBLs）. RESTUTS： A total of 2 089 strains of Gram-negative bacillus were detected in our hospital during 2013-2015， among which there were 1 456 strains of enterobacteria （69.70％） and 633 strains of non-fermentative bacteria， mainly involving Escherichia coli， Pseudomonas aeruginosa， Klebsiella pneumoniae， Acinetobacter baumannii and Enterobacter cloacae. A total of 406 strains of carbapenems-resistant bacteria were detected （19.44％）， including 367 strains of non-fermentative bacteria and 39 strains of enterobacteria. The resistant rates of carbapenems-resistant strains to 16 antibiotics were all higher than 50％， but those of non-carbapenems-resistant strains were in relative low level. Except for aztreonam， resistant rates of carbapenems-resistant strains to other 15 antbiotics were all higher than those of  non-carbapenems-resistant strains， with statistical significance （P＜0.05）. A total of 36 strains of producing MBLs were detected （8.87％）， including 13 strains of producing MBLs drug-resistant P. aeruginosa and 23 strains of producing MBLs drug-resistant A. baumannii； producing MBLs drug resistant enterobacteria had not been found. CONCLUSIONS： Gram-negative bacillus are mainly enterobacteria in our hospital； carbapenems-resistant strains are mainly non-fermentative bacteria， resistant rate of them are commonly higher than that of non-drug-resistant strain. The situation of producing MBLs is serious， and enzyme producing strains are mainly non-fermentative bacteria. It is necessary to strengthen drug resistance of pathogen and enzyme producing strain monitoring， avoid the generation and spreading of drug-resistant strains due to irrational use of antibiotics.