OBJECTIVE： To evaluate the improvement and rectification effects about prescription checking and intervention skills of pharmacists. METHODS： By retrospective investigation， 2 417 outpatient prescriptions and 1 698 outpatient prescriptions were respectively selected from some one hospital during Oct.-Dec. 2015 （before improvement and rectification） and Jan.-Mar. 2016 （after improvement and rectification）. According to standards， guidelines and references as Prescription Administrative Policy， Prescription Review Management Standard （trial） and drug instructions， irrational drug use and prescription checking and intervention skills of pharmacists were compared before and after the implementation of rectification measures as optimizing hospital information system， improving comprehensive skills of pharmacists， strengthening pharmacists’ concept， establishing feedback mechanism. RESULTS： After the implementation of rectification measures， unsuitable indications， clinical diagnosis inconsistent with medication， antibiotics use without indications， unsuitable usage and dosage， unsuitable drug combination and irrational drug use were all decreased， but repeated drug use was increased， without statistical significance （P＞0.05）. The number of non-standard prescriptions and unsuitable prescriptions checked by pharmacists were decreased， without statistical significance （P＞0.05）. The incidence of checking missing， excessive checking and checking error were all increased from 2.40％， 0.99％， 3.39％ before improvement and rectification to 0.47％， 0.29％， 0.77％， with statistical significance （P＜0.05）. CONCLUSIONS： The improvement and rectification measures of the hospital can improve comprehensive prescription checking and intervention skills of pharmacists. Irrational drug use should be further standardized.