OBJECTIVE： To provide reference for the development of pharmaceutical care for periconceptional women. METHODS： Probability proportionate to size sampling（PPS）method was adopted to select participants from periconceptional women in Yongchuan District. The participants were investigated by questionnaires about drug use safety knowledge， attitude and behavior. Epidata 3.0 software was used to establish database. The data were analyzed with SPSS 15.0 software. RESULTS： Totally 800 questionnaires were distributed and then 779 valid questionnaires were collected with effective rate of 97.38％. The total awareness rate of knowledge about drug use safety was 57.69％. The awareness rate rise with the increase of educational level （P＜0.01）. The awareness rate was higher in pregnant women than in prepregnant ones， with statistical significance in most of the questions （P＜0.05 or P＜0.01）. For questions about attitude for drug use safety， the positive answer rate was higher in pregnant women than in prepregnant ones， with statistical significance in most of the questions （P＜0.01）. Among 385 pregnant women， only 56.88％ received folic acid following the WHO recommendation. Of 181 pregnant women who had taken medicine， 8.29％ indicated that the physicians or pharmacists did not inform that whether the medicine affected pregnancy.  CONCLUSIONS： Among periconceptional women， awareness rate of drug use safety is in low level and varied by their educational levels. There are difference in awareness rate of drug use safety and attitude between prepregnant women and pregnant woman. Drug use behavior of pregnant woman is to be modified.