OBJECTIVE： To provide reference for rational use of antibiotics in the clinic. METHODS： In retrospective study， the distribution， composition and drug resistance of pathogens in our hospital during Jan.-Dec. in 2015 were analyzed statistically. RESULTS： In 2015， a total of  9 401 specimens were collected in microbiology laboratory of our hospital； pathogens were detected in 1 743 specimens with positive rate of 18.54％； 1 591 strains of pathogens were isolated， mainly from sputum （59.77％）， urine （14.775） and blood sample （8.93％）. Totally 347 strains of Gram-positive bacteria（21.81％）， 991 strains of Gram-negative bacteria （62.29％）， 253 strains of fungus （15.90％）were detected. Top 4 pathogens in the list of amount were Escherichia coli， Klebsiella pneumoniae， Pseudomonas aeruginosa and Staphylococcus aureus. S. aureus， Streptococcus pneumoniae and Staphylococcus haemolyticus were sensitive to vancomycin， linezolid and sodium fusidate， with resistance rate of 0； resistance rates of them to erythromycin were more than 65％. The resistance rate of S. aureus to penicillin was more than 95％， and S. haemolyticus showed high resistance rate to common antibiotics. Drug resistances of E. coli， K. pneumoniae and P. aeruginosa to carbapenems were all lower than 15％， and they were sensitive to polymyxin with resistance rate of 0. Resistance rate of E. coli to quinolones was more than 50％. CONCLUSIONS： The main pathogens are Gram-negative bacteria in our hospital， drug resistance of them are not satisfactory. Drugs most sensitive to main pathogens include vancomycin， linezolid， carbapenems， etc. The inspection of pathogen drug resistance characteristics should be strengthened， and antibiotics should be selected rationally and normatively according to the results of drug sensitivity test.