OBJECTIVE： To establish a method for simultaneous determination of 5 effective components in Luohua zizhu dry extract. METHODS： UPLC-MS/MS was conducted. The separation was performed on an Zorbax Eclipse Plus C18 column with mobile phase of acetonitrile-water （gradient elution） at the flow rate of 0.3 mL/min. The column temperature was set at 40 ℃ and sample size was 2 μL. The analytes were detected in the multiple reaction monitoring （MRM） mode. Nitrogen was used as drying gas and atomized gas. The temperature and flow rate of drying gas were 325 ℃ and 6 L/min. The pressure of atomized gas was 45 psi. The temperature and flow rate of sheath gas were 350 ℃ and 12 L/min. The voltage of capillary were 4 000 V（+） and 3 500 V（-）. The voltage of nozzle was 500 V. RESULTS：The linear ranges of luteoloside，acteoside，quercetin，luteolin and rutin were 0.504 8- 252.4 ng/mL（r＝0.999 9），0.712 4-356.2 ng/mL（r＝0.999 0），0.509 4-254.7 ng/mL（r＝0.996 2），0.303 0-151.5 ng/mL（r＝0.999 8） and 0.602 2-301.1 ng/mL （r＝0.999 6）， respectively. RSDs of precision， stability and reproducibility tests were all less than 3.0％. The limit of quantitation were 0.42，0.87，0.33，0.12，0.76 ng/mL. The recoveries were 97.99％-101.20％ （RSD＝1.3％，n＝6）， 96.50％-101.20％ （RSD＝1.7％，n＝6）， 94.81％-99.34％ （RSD＝1.7％，n＝6）， 97.54％-100.51％ （RSD＝1.2％，n＝6），93.37％-98.70％ （RSD＝1.9％，n＝6）， respectively. CONCLUSIONS： The method is simple， precise， stable and reproducible， and can be used for simultaneous determination of 5 effective components in Luohua zizhu dry extract.