OBJECTIVE： To establish the method for simultaneous determination of heavy metals in Platycodon grandiflorus， and to explore the effects of altitude on the contents of heavy metals. METHODS： The contents of As， Hg， Pb， Cu and Cd were determined by hydride generation， hydride generation method， graphite furnace method， flame spectrometry， graphite furnace method. SPSS 19.0 software was adopted for clustering analysis and correlation analysis. RESULTS： The linear ranges of As， Hg， Pb， Cu and Cd were 0-16 μg/L（r＝0.996 0）， 0-18 μg/L（r＝0.999 6）， 0-50 μg/L（r＝0.999 7）， 0-0.8 μg/L（r＝0.999 0）and 0-4 μg/L（r＝0.998 0）. Detection limit were 1.3×10－3，2.4×10－4，1.9×10－3，0.33，1.8×10－4 mg/kg. Quantitation limit were  4.4×10－3，8.0×10－4，6.2×10－3，1.1，6.0×10－3 mg/kg. RSDs of precision， stability and repeatability tests were all lower than 3.0％. Recoveries were 99.1％-107.1％（RSD＝3.13％，n＝6）， 94.1％-100.5％（RSD＝2.36％，n＝6），98.9％-104.8％（RSD＝1.81％，n＝6）， 92.7％-100.3％（RSD＝2.92％，n＝6）， 96.6％-99.9％（RSD＝1.26％，n＝6）. Samples with similar altitudes were clustered into a class； the contents of heavy metals decreased as the increase of altitude； there was significant correlation among 5 heavy metals. CONCLUSIONS： The method is simple， precise， stable and repeatable， and can be used for simultaneous determination of heavy metals’ contents in P. grandiflorus. The altitude has a certain effect on the quality of P. grandiflorus.