OBJECTIVE： To establish a method for the simultaneous determination of 6 kinds of residual organic solvents in Omeprazole magnesium， such as methanol， isopropanol， acetonitrile，dichloromethane， ethyl acetate and toluenein. METHODS： Headspace GC was adopted. The determination was performed on the column of DB-624 column by temperature programming. The inlet temperature was 200 ℃， and hydrogen flame ionization detector was adopted with the temperature of 250 ℃； nitrogen gas was used as carrier gas with flow rate of 2.0 mL/min； the splitting-radio was 5 ∶ 1， and sample size was 1 mL； the headspace temperature was 80 ℃ and the equilibrium time was 20 min. RESULTS： The linear range were 12.56-628.00 μg/mL for methanol （r＝0.999 7）， 20.22-1011.20 μg/mL for isopropanol （r＝0.999 9）， 1.96-97.76 μg/mL for acetonitrile （r＝0.999 7），3.10-154.88 μg/mL for dichloromethane （r＝0.999 8），20.69-1034.56 μg/mL for ethyl acetate （r＝0.999 8）， and 3.53-176.72 μg/mL for toluene （r＝0.999 8）； the limits of quantitation were 1.00， 0.91， 0.47， 0.93， 0.41 and 0.35 μg/mL respectively； the limits of detection were 0.31， 0.30， 0.14， 0.31， 0.12 and 0.11 μg/mL respectively； RSDs of precision， stability and reproducibility tests were lower than 3％； the recoveries were 94.53％-101.29％ （RSD＝2.15％，n＝9）， 97.78％-103.42％ （RSD＝1.77％，n＝9）， 96.99％-105.76％ （RSD＝2.59％，n＝9）， 96.83％-102.05％ （RSD＝1.86％，n＝9）， 97.98％-101.13％（RSD＝0.88％，n＝9） and 97.80％-102.40％ （RSD＝1.41％，n＝9）. CONCLUSIONS： The method is sensitive and accurate， can be used for the simultaneous determination of 6 kinds of residual organic solvents in Omeprazole magnesium， such as methanol， isopropanol， acetonitrile， dichloromethane， ethyl acetate and toluenein.